ERM proteins regulate cytoskeleton relaxation promoting T cell-APC conjugation
ERM proteins regulate cytoskeleton relaxation promoting T cell-APC conjugation
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DOI：
10.1038/ni1039
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发表时间：
2004-03-01
影响因子：
30.5
通讯作者：
Delon, J
中科院分区：
文献类型：
--
作者：
Faure, S;Salazar-Fontana, LI;Delon, J
在激活过程中，T细胞与抗原提呈细胞联系在一起，这是一个动态的过程，涉及到广泛的紧密膜接触区域的形成，称为免疫突触。将初始抗原识别与这一现象涉及的细胞骨架变化联系起来的分子中间体尚未定义。在这里，我们证明了Ezrin-Radisin-moesin蛋白在抗原识别后通过Vav1-rac1途径迅速失活。由此产生的皮质肌动蛋白细胞骨架从质膜上分离，降低了细胞的刚性，导致更有效的T细胞-抗原呈递细胞结合物的形成。这些发现确定了一条抗原依赖的分子途径，有利于免疫突触的形成和随后有效免疫反应的发展。
During activation, T cells associate with antigen-presenting cells, a dynamic process that involves the formation of a broad area of intimate membrane contact known as the immunological synapse. The molecular intermediates that link initial antigen recognition to the cytoskeletal changes involved in this phenomenon have not yet been defined. Here we demonstrate that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation. These findings identify an antigen-dependent molecular pathway that favors immunological synapse formation and the subsequent development of an effective immune response.