Purification of human induced pluripotent stem cell-derived neural precursors using magnetic activated cell sorting.
Purification of human induced pluripotent stem cell-derived neural precursors using magnetic activated cell sorting.
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DOI：
10.1007/7651_2014_115
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发表时间：
2015-01-01
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影响因子：
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通讯作者：
Diogo, Maria Margarida
中科院分区：
文献类型：
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作者：
Rodrigues, Goncalo M C;Fernandes, Tiago G;Diogo, Maria Margarida
来源于人类诱导多能干细胞(HiPSCs)的神经前体细胞及其神经元后代将在疾病模型、药物筛选试验、中枢神经系统发育研究中发挥重要作用，甚至可能成为再生医学治疗的有价值的细胞。然而，从HiPSCs中获得同质和同步分化的NP群体是具有挑战性的，而且在神经分化后，许多多能干细胞仍然存在于分化的培养中。在这里，我们描述了一种高效而简单的方法，在12天内分化出HiPSC来源的NPs，我们包括一个最终纯化阶段，在这个阶段，使用磁激活细胞分选(MACS)去除Tra-1-60+多能干细胞(PSCs)，使NP群体几乎没有PSCs。
Neural precursor (NP) cells derived from human induced pluripotent stem cells (hiPSCs), and their neuronal progeny, will play an important role in disease modeling, drug screening tests, central nervous system development studies, and may even become valuable for regenerative medicine treatments. Nonetheless, it is challenging to obtain homogeneous and synchronously differentiated NP populations from hiPSCs, and after neural commitment many pluripotent stem cells remain in the differentiated cultures. Here, we describe an efficient and simple protocol to differentiate hiPSC-derived NPs in 12 days, and we include a final purification stage where Tra-1-60+ pluripotent stem cells (PSCs) are removed using magnetic activated cell sorting (MACS), leaving the NP population nearly free of PSCs.