EXTRACTION OF PLANT-PROTEINS FOR TWO-DIMENSIONAL ELECTROPHORESIS
EXTRACTION OF PLANT-PROTEINS FOR TWO-DIMENSIONAL ELECTROPHORESIS
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DOI：
10.1002/elps.1150091106
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发表时间：
1988-11-01
期刊：
影响因子：
2.9
通讯作者：
GRANIER, F
中科院分区：
文献类型：
--
作者：
GRANIER, F
比较了植物蛋白二维电泳的三种不同提取程序：(i) 使用非变性 Tris 缓冲液提取可溶性蛋白，(ii) 变性提取是在高温下十二烷基硫酸钠的存在，除了回收可溶性蛋白之外还可以溶解膜蛋白，以及 (iii) 三氯乙酸-丙酮程序可以直接沉淀总蛋白。
Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction is presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid-acetone procedure allowing the direct precipitation of total proteins.