Knockdown of JARID2 inhibits the proliferation and invasion of ovarian cancer through the PI3K/Akt signaling pathway
Knockdown of JARID2 inhibits the proliferation and invasion of ovarian cancer through the PI3K/Akt signaling pathway
复制标题
敲低JARID2通过PI3K/Akt信号通路抑制卵巢癌的增殖和侵袭
DOI：
10.3892/mmr.2017.7024
复制
发表时间：
2017-09-01
影响因子：
3.4
通讯作者：
Xu, Pengfei
中科院分区：
文献类型：
--
作者：
Cao, Jian;Li, Huiling;Xu, Pengfei
Jumonji蛋白(JARID2)是JmjC结构域蛋白家族中的一员，已被报道在肿瘤的生长和转移中发挥重要作用。然而，JARID2在卵巢癌中的表达模式和作用尚不清楚。因此，本研究旨在探讨JARID2在卵巢癌中的作用及其机制。本研究结果表明，JARID2在人卵巢癌细胞系中表达上调。此外，JARID2的下调显著抑制了人卵巢癌细胞的增殖、迁移、侵袭和上皮-间充质转化。从机制上讲，JARID2的下调降低了卵巢癌细胞磷酸化肌醇肌醇3-激酶(PI3K)和蛋白激酶B(Akt)的蛋白表达水平。综上所述，实验结果表明，JARID2基因的敲除可能通过使PI3K/Akt信号通路失活而抑制体外细胞的增殖、迁移和侵袭。因此，JARID2基因可能成为卵巢癌治疗的潜在靶点。
Protein Jumonji (JARID2), a member of the family of JmjC domain-containing proteins, has been reported to serve an important role in tumor growth and metastasis. However, the expression pattern and role of JARID2 in ovarian cancer remains unclear. Therefore, in the present study, the role of JARID2 in ovarian cancer was investigated, as well as the underlying mechanisms. The results of the present study demonstrated that the expression of JARID2 is upregulated in human ovarian cancer cell lines. Furthermore, downregulation of JARID2 significantly suppressed proliferation, migration, invasion and epithelial-mesenchymal transition in human ovarian cancer cells. Mechanistically, downregulation of JARID2 decreased the protein expression levels of phosphorylated phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt) in ovarian cancer cells. In conclusion the observations suggested that knockdown of JARID2 inhibited proliferation, migration and invasion in vitro through the inactivation of the PI3K/Akt signaling pathway. Therefore, JARID2 may represent a potential therapeutic target for the treatment of ovarian cancer.