Successful RNA extraction from various human postmortem tissues
Successful RNA extraction from various human postmortem tissues
复制标题
DOI：
10.1007/s00414-006-0131-9
复制
发表时间：
2007-03-01
影响因子：
2.1
通讯作者：
Schmidt, Ulrike
中科院分区：
文献类型：
--
作者：
Heinrich, Marielle;Matt, Katja;Schmidt, Ulrike
最近，一些作者描述了RNA降解与死后间隔（PMI）无关的观察结果，而是与其他参数如环境影响和死亡情况相关。因此，出现了这样一个问题，即基因表达分析是否可以成为法医遗传学中有助于确定死因的一个有价值的工具。在我们的研究中，六个人的组织，从六个人的PMI变化之间的15和118小时，用于总RNA提取。使用GAPDH实时测定进行定量，并通过扩增GAPDH转录物的不同片段长度来检查mRNA的质量。在我们的一组样本中，几乎所有PMI中的所有组织都显示出令人满意的结果，而骨骼肌，其次是大脑和心脏，给出了最好的结果。未检测到PMI和RNA降解之间的相关性，因为对于来自具有最长PMI的个体的所有组织观察到非常好的结果。在这项研究中获得的非常有前途的结果提出了希望，在不久的将来，法医调查的几个领域可能会受益于更多的信息，基因表达模式及其与病理结果的相关性。
Recently, several authors described the observation that RNA degradation does not correlate with the postmortem interval (PMI), but rather with other parameters like environmental impact and the circumstances of death. Therefore, the question arose if the analysis of gene expression could be a valuable tool in forensic genetics to contribute to the determination of the cause of death. In our study, six human tissues obtained from six individuals with PMI varying between 15 and 118 h were used for total RNA extraction. Quantification was performed using a GAPDH real-time assay, and the quality of mRNA was checked by amplification of different fragment lengths of the GAPDH transcript. In our set of samples, nearly all tissues in all PMI revealed satisfactory results, while skeletal muscle, followed by brain and heart, gave the best results. No correlation between PMI and RNA degradation could be detected, as very good results were observed for all tissues from the individual with the longest PMI. The highly promising results obtained in this study raise hopes that in the near future several fields of forensic investigation may profit from additional information about gene expression patterns and their correlation with pathological findings.