Postconditioning attenuates myocardial ischemia-reperfusion injury by inhibiting events in the early minutes of reperfusion
Postconditioning attenuates myocardial ischemia-reperfusion injury by inhibiting events in the early minutes of reperfusion
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DOI：
10.1016/j.cardiores.2004.01.006
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发表时间：
2004-04-01
影响因子：
10.8
通讯作者：
Vinten-Johansen, J
中科院分区：
文献类型：
--
作者：
Kin, H;Zhao, ZQ;Vinten-Johansen, J
目的：我们先前表明，短暂的间歇性缺血应用于再灌注开始期间（即，后处理）在犬缺血-再灌注模型中具有心脏保护作用。本研究验证了这一假设，即再灌注（R）的早期分钟，在此期间应用后处理（Post-con）是其心脏保护的关键。方法：麻醉开胸大鼠，结扎左冠状动脉（LCA）30 min，再灌注3 h。所有大鼠随机分为6组：对照组（n = 8）：R时不干预;缺血预处理组（n = 8）：在首次阻断前阻断LCA 5 min，然后再阻断10 min;术后2（n = 8）：在R的前2分钟内应用6个周期的10 s R和10 s再闭塞;延迟术后（n = 8）：在R的第1分钟内松开结扎线以实现完全复流，之后应用3个周期的术后算法;假手术（n = 6）：手术过程与其他组相同，但不结扎LCA结扎线。结果如下：通过血浆肌酸激酶活性（18 +/- 2* vs. 46 +/- 6 IU/g蛋白）证实，Postcon 1（40 +/- 2%*）中的细胞大小（TTC染色）比对照组（52 +/- 3%）小23%。6个周期的后处理没有进一步减少梗死面积（40 +/-2.9%，p>0.05 vs.后处理1）。同时，IPC组的梗死面积缩小（17 ± 3%）显著大于Post-con 1组（p
Objective: We previously showed that brief intermittent ischemia applied during the onset of reperfusion (i.e., postconditioning) is cardioprotective in a canine model of ischemia-reperfusion. This study tested the hypothesis that the early minutes of reperfusion (R) during which postconditioning (Post-con) is applied are critical to its cardioprotection. Methods: In anesthetized open-chest rats, the left coronary artery (LCA) was occluded for 30 min and reperfused for 3 h. All rats were randomly divided into six groups: Control (n = 8): no intervention at R; Ischemic preconditioning (IPC) (n = 8): the LCA was occluded for 5 min followed by 10 min of R before the index occlusion; Post-con 1 (n = 8): after LCA occlusion, three cycles of 10 s R followed by 10 s LCA re-occlusion were applied during the first minute of R; Post-con 2 (n = 8): Six cycles of 10 s R and 10 s re-occlusion were applied during the first 2 min of R; Delayed Post-con (n = 8): the ligature was loosened for full reflow for the first minute of R, after which the three-cycle Post-con algorithm was applied; Sham (n = 6): the surgical procedure was identical to other groups, but the LCA ligature was not ligated. Results: Infarct size (TTC staining) was 23% smaller in Postcon 1 (40 +/- 2%*) than in Control (52 +/- 3%), confirmed by plasma creatine kinase activity (18 +/- 2* vs. 46 +/- 6 IU/g protein). There was no further reduction in infarct size with 6 cycles of Post-con (40 +/- 2.9%, p>0.05 vs. Post-con 1). Meanwhile, infarct size reduction was significantly greater in the IPC group (17 +/- 3%) than in Post-con 1 (p