Properties of a versatile nanoparticle platform contrast agent to image and characterize atherosclerotic plaques by magnetic resonance imaging
Properties of a versatile nanoparticle platform contrast agent to image and characterize atherosclerotic plaques by magnetic resonance imaging
复制标题
DOI：
10.1021/nl061498r
复制
发表时间：
2006-10-11
期刊：
影响因子：
10.8
通讯作者：
Fisher, Edward A.
中科院分区：
文献类型：
--
作者：
Frias, Juan C.;Ma, Yanqing;Fisher, Edward A.
磁共振成像需要更特异性和选择性的造影剂，促使我们制备一种新的基于高密度脂蛋白（HDL）的纳米颗粒剂。这种第二代造影剂可以以三种不同的方式制备。通过FPLC和凝胶电泳对HDL纳米粒（rHDL）进行了全面表征。该平台的灵活性还允许我们将光学探针掺入rHDL中，通过共聚焦荧光显微镜进行离体定位。将含有造影剂的纳米颗粒注射到发生动脉粥样硬化病变的小鼠体内。动物的磁共振成像显示动脉粥样硬化斑块明显增强。
The need for more specific and selective contrast agents for magnetic resonance imaging motivated us to prepare a new nanoparticle agent based on high-density lipoproteins (HDL). This second generation contrast agent can be prepared in three different ways. The HDL nanoparticles (rHDL) were fully characterized by FPLC and gel electrophoresis. The flexibility of the platform also allows us to incorporate optical probes into rHDL for localization ex vivo by confocal fluorescence microscopy. The contrast-agent-containing nanoparticles were injected into mice that develop atherosclerotic lesions. Magnetic resonance imaging of the animals showed clear enhancement of the atherosclerotic plaques.