Seasonal plasticity and diel stability of H3K27me3 in natural fluctuating environments
Seasonal plasticity and diel stability of H3K27me3 in natural fluctuating environments
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DOI：
10.1038/s41477-020-00757-1
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发表时间：
2020-08-31
期刊：
影响因子：
18
通讯作者：
Kudoh, Hiroshi
中科院分区：
文献类型：
--
作者：
Nishio, Haruki;Nagano, Atsushi J.;Kudoh, Hiroshi
昼夜和季节波动是自然界两大环境变化。虽然生物体通过生理时钟的机制来应对前者（1,2），但对后者长期反应的分子机制的信息有限（3-5）。组蛋白H3赖氨酸27三甲基化（H3K27me3）是一种抑制性组蛋白修饰，在发育转变过程中赋予基因调控的稳定性和可塑性（6-9）。本研究在多年生拟南芥自然种群中研究了H3K27me3在全基因组水平上的季节性和日动态，并将这些动态与组蛋白H3赖氨酸4三甲基化（H3K4me3）的动态进行了比较，H3K4me3是一种活性组蛋白修饰。染色质免疫沉淀测序显示，H3K27me3具有季节性可塑性和细胞稳定性。此外，我们发现季节性H3K27me3振荡相对于H3K4me3振荡在相位上延迟，特别是与环境记忆相关的基因。我们的研究结果表明，H3K27me3可以监测过去的转录活性，从而在自然波动环境中持续数周的生物体反应中形成长期的基因表达趋势。拟南芥中两种组蛋白修饰的全基因组动态表明，H3K27me3具有季节性可塑性和昼夜稳定性，而H3K4me3具有季节性和昼夜变化。H3K27me3相对于H3K4me3有一个延迟相位的季节性振荡。
Diel and seasonal oscillations are two major environmental changes in nature. While organisms cope with the former by the well-characterized mechanism of the circadian clock(1,2), there is limited information on the molecular mechanisms underlying long-term responses to the latter(3-5). Histone H3 lysine 27 trimethylation (H3K27me3), a repressive histone modification, imparts stability and plasticity to gene regulation during developmental transitions(6-9). Here we studied the seasonal and diel dynamics of H3K27me3 at the genome-wide level in a natural population of perennialArabidopsis halleriand compared these dynamics with those of histone H3 lysine 4 trimethylation (H3K4me3), an active histone modification. Chromatin immunoprecipitation sequencing revealed that H3K27me3 exhibits seasonal plasticity and diel stability. Furthermore, we found that the seasonal H3K27me3 oscillation is delayed in phase relative to the H3K4me3 oscillation, particularly for genes associated with environmental memory. Our findings suggest that H3K27me3 monitors past transcriptional activity to create long-term gene expression trends during organismal responses over weeks in natural fluctuating environments.The genome-wide dynamics of two histone modifications inArabidopsis hallerishow that H3K27me3 exhibits seasonal plasticity and diel stability, whereas H3K4me3 changes seasonally and diurnally. H3K27me3 oscillates seasonally with a delayed phase relative to H3K4me3.