Peripheral blood B-cell death compensates for excessive proliferation in lymphoid tissues and maintains homeostasis in bovine leukemia virus-infected sheep
Peripheral blood B-cell death compensates for excessive proliferation in lymphoid tissues and maintains homeostasis in bovine leukemia virus-infected sheep
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DOI：
10.1128/jvi.01022-06
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发表时间：
2006-10-01
影响因子：
5.4
通讯作者：
Willems, Luc
中科院分区：
文献类型：
--
作者：
Debacq, Christophe;Gillet, Nicolas;Willems, Luc
淋巴细胞群体的大小主要由细胞增殖和死亡之间的动态平衡决定。因此，外周血液和淋巴组织之间的淋巴细胞再循环是维持细胞内稳态的关键决定因素。对这些机制的洞察可以从大型动物模型中收集到，在这些模型中，从单个淋巴结进行淋巴管插管是可能的。在这项研究中，我们评估了牛白血病病毒(BLV)感染的绵羊体内淋巴细胞的运输。用羧基荧光素二醋酸酯琥珀酰亚胺酯标记技术，我们证明了淋巴细胞再循环的动力学没有改变，但淋巴组织中加速的增殖可以通过外周血细胞群体中增加的死亡来补偿。因此，在两个不同的组织中，淋巴细胞的动态平衡是通过两个不同的组织中的双相动力学来维持的，强调在BLV感染期间的一个非常动态的过程。
The size of a lymphocyte population is primarily determined by a dynamic equilibrium between cell proliferation and death. Hence, lymphocyte recirculation between the peripheral blood and lymphoid tissues is a key determinant in the maintenance of cell homeostasis. Insights into these mechanisms can be gathered from large-animal models, where lymphatic cannulation from individual lymph nodes is possible. In this study, we assessed in vivo lymphocyte trafficking in bovine leukemia virus (BLV)-infected sheep. With a carboxyfluorescein diacetate succinimidyl ester labeling technique, we demonstrate that the dynamics of lymphocyte recirculation is unaltered but that accelerated proliferation in the lymphoid tissues is compensated for by increased death in the peripheral blood cell population. Lymphocyte homeostasis is thus maintained by biphasic kinetics in two distinct tissues, emphasizing a very dynamic process during BLV infection.