Tracking the Host Response to Infection in Peritoneal Models of Acute Resolving Inflammation.
Tracking the Host Response to Infection in Peritoneal Models of Acute Resolving Inflammation.
复制标题
跟踪急性消退性炎症腹膜模型中宿主对感染的反应。
DOI：
10.1007/978-1-0716-3331-1_7
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Millrine D
中科院分区：
文献类型：
--
作者：
Millrine D
抗菌宿主防御依赖于炎症细胞在感染部位的快速招募、入侵病原体的消除以及炎症的有效消解，从而将对宿主的损害降至最低。腹膜腔提供了一个可接近的和生理上相关的系统，在这里可以研究这些过程的微妙平衡。在这里，我们描述了腹膜炎症的小鼠模型，该模型能够作为反复细菌挑战的结果来研究有效的抗菌素免疫和炎症相关的组织损伤。这些模型的炎症特征反映了腹膜透析肾功能衰竭患者腹膜炎的临床和分子特征。这些模型的发展依赖于从一株表皮葡萄球菌(简称SES)分离出的无细胞培养上清的制备。腹腔注射SES诱导Toll样受体2驱动的急性炎症反应，其特征是最初的中性粒细胞瞬时流入，随后被更持久的单核细胞和淋巴细胞的募集所取代。通过重复使用SES来适应这一模型，可以研究获得性免疫的发展以及与组织重构和纤维化相关的特征。因此，这些模型具有临床意义，为研究先天性和获得性免疫以及间质组织对细菌感染和随之而来的炎症反应的反应提供了令人兴奋的机会。
Antimicrobial host defense is dependent on the rapid recruitment of inflammatory cells to the site of infection, the elimination of invading pathogens, and the efficient resolution of inflammation that minimizes damage to the host. The peritoneal cavity provides an accessible and physiologically relevant system where the delicate balance of these processes may be studied. Here, we describe murine models of peritoneal inflammation that enable studies of competent antimicrobial immunity and inflammation-associated tissue damage as a consequence of recurrent bacterial challenge. The inflammatory hallmarks of these models reflect the clinical and molecular features of peritonitis seen in renal failure patients on peritoneal dialysis. The development of these models relies on the preparation of a cell-free supernatant derived from an isolate ofStaphylococcus epidermidis(termed SES). Intraperitoneal administration of SES induces a Toll-like receptor 2-driven acute inflammatory response that is characterized by an initial transient influx of neutrophils that are replaced by a more sustained recruitment of mononuclear cells and lymphocytes. Adaptation of this model using a repeated administration of SES allows investigations into the development of adaptive immunity and the hallmarks associated with tissue remodelling and fibrosis. These models are therefore clinically relevant and provide exciting opportunities to study innate and adaptive immunity and the response of the stromal tissue compartment to bacterial infection and the ensuing inflammatory reaction.
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影响因子：
27.4
作者：
Jones GW;Greenhill CJ;Williams JO;Nowell MA;Williams AS;Jenkins BJ;Jones SA
通讯作者：
Jones SA
影响因子：
4.4
作者：
McLoughlin, RM;Hurst, SM;Jones, SA
通讯作者：
Jones, SA
DOI：
10.1074/jbc.m109.021634
发表时间：
2009-08-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Morgan AH;Dioszeghy V;Maskrey BH;Thomas CP;Clark SR;Mathie SA;Lloyd CM;Kühn H;Topley N;Coles BC;Taylor PR;Jones SA;O'Donnell VB
通讯作者：
O'Donnell VB
制瘤素 M 受体 -b 信号传导限制急性炎症中单核细胞的募集。
DOI：
--
发表时间：
2008
期刊：
J. Immunol. 181(3)
影响因子：
--
作者：
Hams E;Colmont C. S.;Dioszeghy V.;Hammond V. J.;Fielding C. A.;Williams A. S.;Tanaka M.;Miyajima A.;Taylor P. R.;Topley N.;Jones S. A.
通讯作者：
Jones S. A.