Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery.
Washed preparation of faecal microbiota changes the transplantation related safety, quantitative method and delivery.
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粪便微生物群的洗涤制剂改变了移植相关的安全性、定量方法和递送
DOI：
10.1111/1751-7915.14074
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发表时间：
2022-09
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
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作者：
在实践中，不同国家的粪便微生物群移植（FMT）的安全性、定量方法和实施方式存在很大差异。近年来，基于自动过滤、洗涤过程和相关递送的FMT改进方法被命名为洗涤微生物群移植（washed microbiota transplantation，WMT）。首先，本研究旨在描述2012年至2021年中国微生物群移植系统（CMTS）（一家提供全国性非营利性服务的集中粪便库）从手工到冲洗制备的FMT方法学发展。次要目的是描述供体筛查、粪便重量与治疗剂量之间的相关性、不良事件的发生率和分娩决定。对前瞻性记录的数据进行回顾性分析。结果：供者筛选成功率为3.1%（32/1036）。溃疡性结肠炎（UC）患者的发热发生率从手工FMT的19.4%（6/31）显著降低到WMT的2.7%（24/902），这使得UC成为反映粪便菌群制剂质量控制的一个相当可观的疾病模型。我们根据富集的微生物群而不是粗略的粪便重量将一个处理单位定义为10 cm 3微生物群沉淀（1.0 × 1013个细菌）。对于递送微生物群，结肠经内镜肠内管是一种有前途的方式，特别是对于多次WMT或频繁结肠给药与WMT联合使用。这项研究将有助于改善FMT的更好实践，以帮助更多的患者在未来。在实践中，不同国家的粪便微生物群移植（FMT）的安全性、定量方法和实施方式存在很大差异。近年来，基于自动过滤、洗涤过程和相关递送的FMT改进方法被命名为洗涤微生物群移植（washed microbiota transplantation，WMT）。2012年至2021年，中国微生物群移植系统（CMTS）描述了FMT从手工制备到冲洗制备的方法学发展，CMTS是一家提供国家非营利性服务的集中粪便库。这项研究将有助于改善FMT的更好实践，以帮助更多的患者在未来。
The safety, quantitative method and delivery of faecal microbiota transplantation (FMT) vary a lot from different countries in practice. Recently, the improved methodology of FMT based on the automatic filtration, washing process and the related delivery was named as washed microbiota transplantation (WMT). First, this study aimed to describe the methodology development of FMT from manual to washing preparation from 2012 to 2021 in China Microbiota Transplantation System (CMTS), a centralized stool bank for providing a national non‐profit service. The secondary aim is to describe donor screenings, the correlation between faecal weight and treatment doses, incidence of adverse events and delivery decision. The retrospective analysis on the prospectively recorded data was performed. Results showed that the success rate of donor screening was 3.1% (32/1036). The incidence rate of fever decreased significantly from 19.4% (6/31) in manual FMT to 2.7% (24/902) in WMT in patients with ulcerative colitis (UC), which made UC a considerable disease model to reflect the quality control of faecal microbiota preparation. We defined one treatment unit as 10 cm3 microbiota precipitation (1.0 × 1013 bacteria) based on enriched microbiota instead of rough faecal weight. For delivering microbiota, colonic transendoscopic enteral tube is a promising way especially for multiple WMTs or frequent colonic administration of drugs combined with WMT. This study should help improve the better practice of FMT for helping more patients in the future. The safety, quantitative method and delivery of faecal microbiota transplantation (FMT) vary a lot from different countries in practice. Recently, the improved methodology of FMT based on the automatic filtration, washing process and the related delivery was named as washed microbiota transplantation (WMT). The methodology development of FMT from manual to washing preparation was described from 2012 to 2021 in China Microbiota Transplantation System (CMTS), a centralized stool bank for providing a national non‐profit service. This study should help improve the better practice of FMT for helping more patients in the future.