Quantifying gluconeogenesis during fasting
Quantifying gluconeogenesis during fasting
复制标题
DOI：
10.1152/ajpendo.1997.273.6.e1209
复制
发表时间：
1997-12-01
影响因子：
5.1
通讯作者：
Landau, BR
中科院分区：
文献类型：
--
作者：
Chandramouli, V;Ekberg, K;Landau, BR
在三组健康受试者进行性禁食期间，研究了(H2O)-H-2在评估糖异生对葡萄糖生成的贡献(%GNG)方面的作用。一组(n=3)摄入(H2O)-H-2，使体内水浓缩约为0.35%，5h内禁食。用六亚甲基四胺测定血糖C-5和C-2位氢气的浓集比，每隔2小时测定GNG%。GNG的百分比从10h的40+/-8%增加到42h的93+/-6%。另一组在2.25h内摄取(H2O)-H-2，从11h(n=7)和19h(n=7)开始，达到接近0.5%的水体富集度。在(H2O)-H-2摄入完成后约1h，在血浆水中和在C-2中的浓缩达到稳定状态。C-5/C-2比值在(H2O)-H-2摄取完成后达到稳定状态。%GNG在14h时为54+/-2%，在22h时为+/-2%。3 h[6，6-H-2(2)]葡萄糖输注也开始估计C-6富集葡萄糖的产量，同样是在六亚甲基四胺中。糖异生产生的葡萄糖在14和22小时都是0.99+/-0.06毫克·公斤(-1)·分(-1)。在第三组(n=3)中，GNG在摄取(H2O)-H-2后大约2小时达到稳定状态，仅接近0.25%的浓缩。[n结论]摄取(H2O)-H-2后2小时的GNG和使用[6，6-H-2(2)]输注葡萄糖产生的葡萄糖可以从血糖C-2、C-5、和C-6。%GNG从吸收后至禁食42h逐渐增加，14和22h糖异生产生的葡萄糖量无明显变化。
The use of (H2O)-H-2 in estimating gluconeogenesis' contribution to glucose production (%GNG) was examined during progressive fasting in three groups of healthy subjects. One group (n = 3) ingested (H2O)-H-2 to a body water enrichment of approximate to 0.35% 5 h into the fast. %GNG was determined at 2-h intervals from the ratio of the enrichments of the hydrogens at C-5 and C-2 of blood glucose, assayed in hexamethylenetetramine. %GNG increased from 40 +/- 8% at 10 h to 93 +/- 6% at 42 h. Another group ingested (H2O)-H-2 over 2.25 h, beginning at 11 h (n = 7) and 19 h (n = 7) to achieve approximate to 0.5% water enrichment. Enrichment in plasma water and at C-2 reached steady state approximate to 1 h after completion of (H2O)-H-2 ingestion. The C-5-to-C-2 ratio reached steady state by the completion of (H2O)-H-2 ingestion. %GNG was 54 +/- 2% at 14 h and 64 +/- 2% at 22 h. A 3-h [6,6-H-2(2)]glucose infusion was also begun to estimate glucose production from enrichments at C-6, again in hexamethylenetetramine. Glucose produced by gluconeogenesis was 0.99 +/- 0.06 mg.kg(-1).min(-1) at both 14 and 22 h. In a third group (n = 3) %GNG reached steady state approximate to 2 h after (H2O)-H-2 ingestion to only approximate to 0.25% enrichment.:[n conclusion, %GNG by 2 h after (H2O)-H-2 ingestion and glucose production using [6,6-H-2(2)]glucose infusion, begun together, can be determined from hydrogen enrichments at blood glucose C-2, C-5, and C-6. %GNG increases gradually from the postabsorptive state to 42 h of fasting, without apparent change in the quantity of glucose produced by gluconeogenesis at 14 and 22 h.