Novel Three-dimensional Micro Vibration Actuator for Imposing Dynamic Stimulations to Promote Differentiation of iPS Cells
Novel Three-dimensional Micro Vibration Actuator for Imposing Dynamic Stimulations to Promote Differentiation of iPS Cells
复制标题
新型三维微振动致动器施加动态刺激以促进 iPS 细胞分化
DOI：
10.1007/s00542-015-2644-y
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Kaoru Goto
中科院分区：
文献类型：
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作者：
Tadashi Kosawada;Tomoyuki Koizumi;Kazuya Ugajin;Zhonggang Feng;Kaoru Goto
在再生医学的最近进展中，强烈需要开发支持微小活细胞的主动动态致动的装置。本研究利用有限元素法分析，开发出一种简单而有效的三维微振动驱动器，可在有限的空间与环境下，应用于倒置相差显微镜系统，直接刺激培养的iPS细胞集落，促进其分化为目标细胞。开发的致动器使我们能够在其微探针中激发三维振动，在每个X，Y和Z方向上的总位移幅度高达30 µm。为了评估致动器的效果，小鼠iPS细胞集落用10 μm的压痕以及频率为1、5、10、15 Hz的剪切振动进行刺激。通过测定诱导分化的神经细胞β Ⅲ-微管蛋白和F-肌动蛋白的免疫染色面积，研究了这些动态刺激对小鼠iPS细胞分化过程的影响。结果发现，静态培养的小鼠iPS细胞向神经元分化的效率与动态刺激的小鼠iPS细胞分化的效率存在显著差异。此外，证实了动态刺激的模式对促进小鼠iPS细胞分化为神经元具有决定性影响。
In recent advancement of regenerative medicine, it is strongly needed to develop devices to support active dynamic actuation of minute living cells. In this study, with the aid of finite element method analysis, simple but effective cantilever-type three-dimensional micro vibration actuator to be used within limited space and environment on inverted phase-contrast microscope system is newly developed to stimulate cultured living iPS cells colony directly to promote their differentiation into objective cells. The developed actuator enables us to excite three-dimensional vibrations in its micro-probe with total amplitude of displacement up to 30 µm in each X, Y and Z direction. In order to estimate effect of the actuator, mouse iPS cells colony is stimulated with 10 µm indenting as well as shearing vibrations with frequencies of 1, 5, 10, 15 Hz. Effects of these dynamic stimulations on the differentiation process of the mouse iPS cells are investigated by measuring the immunostained areas of βIII-tubulin and F-actin of the differentiated neural cells with or without the dynamic stimulation. It is found that there is significant difference in differentiation efficiency of the mouse iPS cells into neurons between the statically cultured cells and the dynamically stimulated ones. Also, it is confirmed that the mode of the dynamic stimulation has a decisive influence to promote the differentiation of mouse iPS cells into neurons.