Microbial communities on fish eggs from Acanthopagrus schlegelii and Halichoeres nigrescens at the XuWen coral reef in the Gulf of Tonkin
Microbial communities on fish eggs from Acanthopagrus schlegelii and Halichoeres nigrescens at the XuWen coral reef in the Gulf of Tonkin
复制标题
北部湾徐文珊瑚礁中的棘鲢和黑鲨鱼卵上的微生物群落
DOI：
10.7717/peerj.8517
复制
发表时间：
2020-02-07
期刊：
影响因子：
2.7
通讯作者：
Hou, Gang
中科院分区：
文献类型：
--
作者：
Bai, Shijie;Hou, Gang
珊瑚礁是海洋生态系统的重要组成部分，也是海洋鱼类的重要产卵场。微生物在这种环境中非常丰富，在宿主物种的生长和发育中起着关键作用。许多研究调查了鱼类的微生物群落，重点是实验室饲养的成鱼的肠道微生物组。人们对鱼卵和其微生物之间的关系知之甚少，特别是微生物群落与珊瑚礁中的野生鱼卵有关。本研究利用16 S rRNA基因扩增测序技术，对两种珊瑚鱼卵--许氏黄颡鱼和黑海猪鱼卵的微生物群落进行了分析。假单胞菌属、无色杆菌属和沙雷氏菌属是与这些鱼卵相关的主要细菌属，并且已知是具有潜在致病性和腐败作用的细菌。根据30个最丰富的操作分类单位（OTU），对许氏黄颡鱼和黑海猪卵的微生物群落结构进行了分离。主坐标分析（PCoA）和非度量多维标度分析（NMDS）进一步证实了珊瑚鱼卵微生物群落的物种差异，这可能是由于宿主选择。对微生物群落的功能预测表明，大部分微生物群落是化能异养的，并参与氮循环。我们的研究结果表明，珊瑚鱼卵的微生物群落是不同的物种和关键的微生物是潜在的致病性，导致鱼卵的腐败，高死亡率，低孵化率。该研究为了解微生物与野生鱼卵的关系提供了新的见解。
Coral reefs are an important part of the ocean ecosystem and are a vital spawning ground for marine fish. Microorganisms are abundant in this environment and play a key role in the growth and development of host species. Many studies have investigated the microbial communities of fish with a focus on the intestinal microbiome of laboratory-reared adult fish. Little is known about the relationship between fish eggs and their microorganisms, especially as microbial communities relate to wild fish eggs in coral reefs. In this study, we analyzed the microbial communities of two species of coral fish eggs, Acanthopagrus schlegelii and Halichoeres nigrescens, using 16S rRNA gene amplicon sequencing technology. Pseudomonas, Archromobacter, and Serratia were the main bacterial genera associated with these fish eggs and are known to be bacteria with potentially pathogenic and spoilage effects. The microbial community structures of Acanthopagrus schlegelii and Halichoeres nigrescens eggs were separated based on the 30 most abundant operational taxonomic units (OTUs). Principal coordinate analysis (PCoA) and non-metric multidimensional scaling analysis (NMDS) further confirmed that the microbial communities of coral fish eggs differ by species, which may be due to host selection. A functional prediction of the microbial communities indicated that most of the microbial communities were chemoheterotrophic and involved in nitrogen cycling. Our results showed that the microbial communities of coral fish eggs were distinct by species and that key microorganisms were potentially pathogenic, leading to the spoilage of fish eggs, high mortality, and low incubation rates. This study provided new insights for understanding the relationship between microorganisms and wild fish eggs.