A comparative study of two angiogenic factors - Vascular endothelial growth factor and angiogenin in induced sputum from asthmatic children in acute attack
A comparative study of two angiogenic factors - Vascular endothelial growth factor and angiogenin in induced sputum from asthmatic children in acute attack
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DOI：
10.1378/chest.129.2.266
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发表时间：
2006-02-01
期刊：
影响因子：
9.6
通讯作者：
Bakr, SI
中科院分区：
文献类型：
--
作者：
Abdel-Rahman, AMO;El-Sahrigy, SAF;Bakr, SI
背景：血管生成是支气管哮喘气道重构的先决条件。一些因素可能通过影响炎症细胞浸润或新生血管在炎症和血管生成中发挥重要作用。目的：(1)测定哮喘患儿急性发作时及治疗开始后6周痰上清中血管内皮生长因子（VEGF）和血管生成素水平；(2)它们的水平与哮喘严重程度的相关性。研究对象和方法：20例急性发作哮喘患儿（平均年龄9.6 +/- 3.5岁[+/- SD]）和12例性别和年龄匹配的健康对照儿童。收集痰上清液，测定VEGF和血管生成素水平。抽取血清进行IgE检测。上述试验采用酶联免疫吸附法进行。肺活量法测定FEV1。在治疗开始6周后，对哮喘患儿重复进行VEGF、血管生成素和FEV1的评估。结果：哮喘患儿急性发作时血管内皮生长因子和血管生成素水平明显高于正常对照组（p < 0.001）。VEGF和血管生成素水平随哮喘严重程度的增加而升高（p < 0.001）。两种血管生成因子之间存在显著正相关（r = 0.98, p < 0.001）。预测FEV1百分比与VEGF （r = - 0.99, p < 0.001）、angiogenin （r = - 0.97, p < 0.001）呈显著负相关。血清IgE与痰中VEGF无显著相关性（r = 0.09, p < 0.05）。虽然经皮质类固醇吸入治疗6周后VEGF和血管生成素水平均有显著下降，但未达到正常对照水平（p < 0.001和p < 0.05）。结论：我们的研究结果表明，急性哮喘患儿的VEGF和血管生成素水平均升高。该研究还表明，儿童支气管哮喘严重程度的增加与两种血管生成因子的表达有关，这两种因子与哮喘发病机制有关。治疗6周后，两种血管生成因子水平均显著降低。
Background: Angiogenesis is a prerequisite for airway remodeling in bronchial asthma. Several factors may play important roles in inflammation and angiogenesis through effects on inflammatory cell infiltration or neovascularization.Objectives: (1) To determine the levels of vascular endothelial growth factor (VEGF) and angiogenin in sputum supernatants of asthmatic children during the acute attack and 6 weeks after start of therapy; and (2) to correlate their levels with the degree of asthma severity.Subjects and methods: Twenty asthmatic children with acute attack (mean age, 9.6 +/- 3.5 years [+/- SD]) and 12 sex- and age-matched healthy control children were enrolled in the study. Sputum supernatants were collected for determination of VEGF and angiogenin levels. Serum samples were withdrawn for IgE measurement. The above tests were performed using an enzyme-linked immunosorbent assay. The FEV1 was measured using spirometry. VEGF, angiogenin, and FEV1 estimations were repeated for asthmatic children 6 weeks after start of therapy. Results: During the acute attack, asthmatic children had significantly higher levels of VEGF and angiogenin than in healthy control children (p < 0.001). VEGF and angiogenin levels showed more elevation with increase in asthma severity (p < 0.001). A significant positive correlation existed between both angiogenic factors (r = 0.98, p < 0.001). A negative significant correlation was found between FEV1 percentage of predicted and both VEGF (r = - 0.99, p < 0.001) and angiogenin (r = - 0.97, p < 0.001). A nonsignificant correlation was found between serum IgE and sputum VEGF (r = 0.09, p > 0.05). Although there was a significant decrease in the levels of both VEGF and angiogenin after 6 weeks of treatment with corticosteroid inhalation therapy, the levels did not reach normal control levels (p < 0.001 and p < 0.05, respectively).Conclusions: Our results show that both VEGF and angiogenin levels were elevated in children with acute asthma. The study also suggests that increased severity of bronchial asthma in children is associated with the expression of both angiogenic factors, which are implicated in asthma pathogenesis. After 6 weeks of therapy, the levels of both angiogenic factors showed significant decrease.