Activation of ethylene-related genes in response to aphid feeding on resistant and susceptible melon and tomato plants
Activation of ethylene-related genes in response to aphid feeding on resistant and susceptible melon and tomato plants
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DOI：
10.1111/j.1570-7458.2009.00945.x
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发表时间：
2010-02-01
影响因子：
1.9
通讯作者：
Goggin, Fiona
中科院分区：
文献类型：
--
作者：
Anstead, James;Samuel, Preethi;Goggin, Fiona
乙烯（ET）是一种简单的气体化合物，长期以来一直被认为是植物对昆虫取食和病原体攻击反应的共同组分。然而，目前还不确定它是否在寄主植物对刺吸式昆虫如蚜虫的抗性中起作用。在这些实验中，我们研究了两个模型系统中的关键ET相关基因的表达的抗和感的相互作用：番茄-Mi-Macrosiphum euphorphium（托马斯）（半翅目：蚜虫科：Macrosiphini）系统和甜瓜病毒蚜虫传递基因（Vat）-Aphis gossypii格洛弗（半翅目：蚜虫：Aphidini）系统。我们研究了与ET合成、感知、信号转导和下游反应相关的基因的表达模式。与对照植物相比，蚜虫侵染的植物在基因表达上表现出显着差异。特别是ET信号通路基因和下游反应基因在A. Gossypii和Vat+的抗性，表明ET可能在Vat介导的寄主植物抗性中发挥作用。ET和茉莉酸途径（Cm-ERF 1）之间的关键集成器显示出最强的响应。
The simple gaseous compound ethylene (ET) has long been recognized as a common component of plant responses to insect feeding and pathogen attack. However, it is presently uncertain whether it plays a role in host-plant resistance to piercing-sucking insects such as aphids. In these experiments, we investigated the expression of key ET-associated genes in resistant and susceptible interactions in two model systems: the tomato-Mi-Macrosiphum euphorbiae (Thomas) (Hemiptera: Aphididae: Macrosiphini) system and the melon-virus aphid transmission gene (Vat)-Aphis gossypii Glover (Hemiptera: Aphididiae: Aphidini) system. We examined expression patterns of genes associated with ET synthesis, perception, signal transduction, and downstream response. When compared with control plants, plants infested with aphids showed marked differences in gene expression. In particular, ET signaling pathway genes and downstream response genes were highly upregulated in the resistant interaction between A. gossypii and Vat+, indicating ET may play a role in Vat-mediated host-plant resistance. A key integrator between the ET and jasmonic acid pathways (Cm-ERF1) showed the strongest response.