Differential expression of the mouse homeobox-containing gene Hox-1.4 during male germ cell differentiation and embryonic development.
Differential expression of the mouse homeobox-containing gene Hox-1.4 during male germ cell differentiation and embryonic development.
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小鼠同源盒基因 Hox-1.4 在雄性生殖细胞分化和胚胎发育过程中的差异表达。
DOI：
10.1073/pnas.84.16.5813
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发表时间：
1987
影响因子：
11.1
通讯作者：
Martin,GR
中科院分区：
文献类型：
--
作者：
Wolgemuth,DJ;Viviano,CM;Gizang-Ginsberg,E;Frohman,MA;Joyner,AL;Martin,GR
Hox-1.4是一种含有小鼠同源盒的基因（最初鉴定为HBT-1），其表达在成年动物中似乎具有睾丸特异性。对缺乏生殖细胞的突变小鼠睾丸RNA中Hox-1.4转录本的检测证实，Hox-1.4在睾丸中的表达是生殖细胞特异性的。研究人员利用丰富的生精细胞群体来定位Hox-1.4在进入减数分裂前期分化和分化后的生殖细胞中的特异性表达，并证实存在两种不同大小的Hox-1.4转录本。对畸胎瘤细胞培养物和妊娠10.5-16.5天小鼠胚胎的RNA进行检查，发现存在几种Hox-1.4转录本，比生殖细胞中存在的转录本要大。在妊娠中期胎儿中，Hox-1.4在脊髓中表达最为丰富。
Hox-1.4 is a mouse homeobox-containing gene (initially identified as HBT-1), whose expression appears to be testis-specific in the adult animal. Examination of Hox-1.4 transcripts in RNA from testes of mutant mice deficient in germ cells confirms that Hox-1.4 expression within the testis is germ cell-specific. Enriched populations of spermatogenic cells were used to localize the expression of Hox-1.4 specifically to germ cells that have entered into and progressed beyond the meiotic prophase stage of differentiation and to demonstrate the presence of two different size Hox-1.4 transcripts. Examination of RNA from teratocarcinoma cell cultures and mouse embryos at 10.5-16.5 days of gestation demonstrated the presence of several Hox-1.4 transcripts, which are larger than those present in germ cells. In the midgestation fetus, Hox-1.4 expression is most abundant in the spinal cord.