Site‐specific responses of foliar fungal microbiomes to nutrient addition and herbivory at different spatial scales
Site‐specific responses of foliar fungal microbiomes to nutrient addition and herbivory at different spatial scales
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DOI：
10.1002/ece3.5711
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发表时间：
2019-10
影响因子：
2.6
通讯作者：
Candice Y. Lumibao;E. Borer;Bradford Condon;L. Kinkel;G. May;E. Seabloom
中科院分区：
文献类型：
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作者：
Candice Y. Lumibao;E. Borer;Bradford Condon;L. Kinkel;G. May;E. Seabloom
植物微生物组可以通过多种方式影响寄主的功能，表征影响内生（寄主植物组织内的微生物）群落多样性的生态因子是了解环境变化对这些群落影响的关键步骤。群落成员之间的系统发育相关性提供了一种量化群落系统发育多样性的方法，并可以深入了解形成内生微生物组的生态因素。在美国中部大平原的4个地点，研究了添加实验营养物和排除草食对杰拉氏Andropogon gerardii禾本科植物叶片内生真菌群落系统发育多样性的影响。利用扩增子测序技术，研究了施肥和草食对大平原真菌群落系统发育多样性的影响。尽管真菌的多样性和丰富度不断增加，但在更大的空间尺度上，真菌微生物组是由具有随机系统发育关联的分类群组成的。从样地内部几米到样地之间数百公里的空间尺度上，系统发育多样性没有系统差异。我们观察到不同地点的组成发生了实质性的变化，表明不同的真菌群落在整个地区的不同地点保持着相似的多样性。相反，在叶片内尺度上，无论环境如何，真菌群落都倾向于由密切相关的分类群组成，但群落之间的系统发育组成没有变化。我们还发现，在不同的地点，营养添加（施肥）和草食有不同的效果。这些结果表明，环境变化的方向和幅度可能取决于所考虑的空间尺度，也可能受到微生物多样性和组成的区域站点差异的限制。
Abstract The plant microbiome can affect host function in many ways and characterizing the ecological factors that shape endophytic (microbes living inside host plant tissues) community diversity is a key step in understanding the impacts of environmental change on these communities. Phylogenetic relatedness among members of a community offers a way of quantifying phylogenetic diversity of a community and can provide insight into the ecological factors that shape endophyte microbiomes. We examined the effects of experimental nutrient addition and herbivory exclusion on the phylogenetic diversity of foliar fungal endophyte communities of the grass species Andropogon gerardii at four sites in the Great Plains of the central USA. Using amplicon sequencing, we characterized the effects of fertilization and herbivory on fungal community phylogenetic diversity at spatial scales that spanned within‐host to between sites across the Great Plains. Despite increasing fungal diversity and richness, at larger spatial scales, fungal microbiomes were composed of taxa showing random phylogenetic associations. Phylogenetic diversity did not differ systematically when summed across increasing spatial scales from a few meters within plots to hundreds of kilometers among sites. We observed substantial shifts in composition across sites, demonstrating distinct but similarly diverse fungal communities were maintained within sites across the region. In contrast, at the scale of within leaves, fungal communities tended to be comprised of closely related taxa regardless of the environment, but there were no shifts in phylogenetic composition among communities. We also found that nutrient addition (fertilization) and herbivory have varying effects at different sites. These results suggest that the direction and magnitude of the outcomes of environmental modifications likely depend on the spatial scale considered, and can also be constrained by regional site differences in microbial diversity and composition.