Gonadotropin-binding sites in the rhesus monkey ovary: role of the vasculature in the selective distribution of human chorionic gonadotropin to the preovulatory follicle.
Gonadotropin-binding sites in the rhesus monkey ovary: role of the vasculature in the selective distribution of human chorionic gonadotropin to the preovulatory follicle.
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DOI：
10.1210/endo-109-2-356
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发表时间：
1981-08
期刊：
影响因子：
4.8
通讯作者：
Anthony J. Zelezni;Harold M. Schuler;Leo E. Reichert
中科院分区：
文献类型：
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作者：
Anthony J. Zelezni;Harold M. Schuler;Leo E. Reichert
这些实验的开始是为了确定在月经周期的卵泡后期，恒河猴卵巢中单个卵泡的血管系统是否存在差异，以及血管系统的差异是否导致某些卵泡对促性腺激素的不同暴露。在月经周期第9天和第10天切除的4只动物卵巢中，测定了优势卵泡和其他窦卵泡鞘层内的血管密度。血管用组织化学染色检测血红蛋白。形态计量学分析表明，优势卵泡鞘膜层被血管占据的比例（48%）显著高于与优势卵泡位于同一卵巢内（24%）或对侧卵巢内（26%）的其他小窦卵泡（P < 0.005）。为了确定血管分布的差异是否导致促性腺激素向优势卵泡的不同供应，我们通过放射自显影研究了4只恒河猴在月经周期第9天体内和体外[125I]hCG的结合。体外结合研究结果表明，几乎每个窦卵泡的鞘层都具有hcg结合位点。然而，当将[125I]hcg静脉注射到动物体内并通过脉管系统分布时，优势卵泡被大量标记，而其他较小的窦腔卵泡即使有，也很少积累放射性hcg。这些观察结果表明，单个卵泡血管化的增加导致促性腺激素的优先递送，并表明单个卵泡的血流量可能在恒河猴排卵前卵泡的选择性成熟中起重要作用。
These experiments were initiated to determine if differences exist in the vasculature of individual follicles in the rhesus monkey ovary during the late follicular phase of the menstrual cycle and to determine whether differences in vascularity result in differential exposure of certain follicles to gonadotropic hormones. The density of blood vessels within the thecal layer of the dominant follicle and other antral follicles was determined in ovaries from four animals removed on day 9 or 10 of the menstrual cycle. Blood vessels were identified using a histochemical stain for hemoglobin. Morphometric analysis indicated that the percentage of the thecal layer occupied by blood vessels in the dominant follicles (48%) was significantly greater (P less than 0.005) than that of other smaller antral follicles either within the same ovary as the dominant follicle (24%) or in the contralateral ovary (26%). To determine if differences in vascularity result in a differential supply of gonadotropins to the dominant follicle, we studied, by autoradiography, the in vivo and in vitro binding of [125I]hCG in four rhesus monkeys on day 9 of the menstrual cycle. Results of in vitro binding studies indicated that the thecal layer of virtually every antral follicle possessed hCG-binding sites. However, when [125I]hcg was injected iv into animals and allowed to distribute via the vasculature, the dominant follicle was heavily labeled while other smaller antral follicles accumulated little, if any, radioiodinated hCG. These observations indicate that increased vascularization of individual follicles results in preferential delivery of gonadotropins, and suggest that blood flow to individual follicles may play an instrumental role in the selective maturation of the preovulatory follicle in the rhesus monkey.