Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis.
Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis.
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DOI：
10.1093/nar/18.5.1255
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发表时间：
1990-03
影响因子：
14.9
通讯作者：
J. Singer-Sam;M. Robinson;A. Bellvé;Melvin l. Simon;A. Riggs
中科院分区：
文献类型：
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作者：
J. Singer-Sam;M. Robinson;A. Bellvé;Melvin l. Simon;A. Riggs
采用逆转录聚合酶链反应法（RT-PCR）定量测定了不同生精阶段小鼠雄性生殖细胞总RNA中RNA转录物的累积水平。两种x连锁酶，磷酸甘油酸激酶（PGK-1）和次黄嘌呤磷酸核糖基转移酶（HPRT）的RNA水平在精子发生过程中都会下降，尽管PGK-1的转录物水平下降得更快。在所有测试的生精细胞中，y连接的ZFY（锌指蛋白）的RNA都升高，在瘦素/zygotene精子细胞和圆形精子细胞中尤其高。腺嘌呤磷酸核糖基转移酶（APRT）的RNA增加5倍，在肺肿晚期达到峰值。PGK-2的RNA，在精原细胞中检测不到，在圆形精细胞阶段增加至少50倍。DNA（胞嘧啶-5-）甲基转移酶（MTase）转录水平在精子发生过程中比在非分裂肝细胞中高出一个数量级。
A reverse transcriptase-polymerase chain reaction assay (RT-PCR) was used quantitatively to measure accumulated levels of RNA transcripts in total mouse RNAs derived from male germ cells at various spermatogenic stages. RNA levels for two X-linked enzymes, phosphoglycerate kinase (PGK-1) and hypoxanthine phosphoribosyl transferase (HPRT), both decrease during spermatogenesis, although the transcript levels decrease much more rapidly for PGK-1. RNA for the Y-linked ZFY (zinc finger protein) is elevated in all spermatogenic cell fractions tested, being particularly high in leptotene/zygotene spermatocytes and round spermatids. RNA for adenine phosphoribosyltransferase (APRT) increases 5-fold to a peak during late pachynema. RNA for PGK-2, undetectable in spermatogonial cells, increases at least 50-fold by the round spermatid stage. DNA (cytosine-5-)-methyltransferase (MTase) transcript levels are over an order of magnitude higher throughout spermatogenesis than in non-dividing liver cells.