Molecular and morphologic changes during the epithelial-mesenchymal transformation of palatal shelf medial edge epithelium in vitro.
Molecular and morphologic changes during the epithelial-mesenchymal transformation of palatal shelf medial edge epithelium in vitro.
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DOI：
10.1387/ijdb.1801871
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发表时间：
1991-12
期刊：
影响因子：
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通讯作者：
C. F. Shuler;Yan Guo;Asima Majumder;Ruiyuan Luo
中科院分区：
文献类型：
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作者：
C. F. Shuler;Yan Guo;Asima Majumder;Ruiyuan Luo
腭融合期间内侧边缘上皮（MEE）细胞的命运被认为是细胞程序性死亡或上皮-间充质转化。采用活细胞标记技术标记腭突，观察其在腭突融合过程中的变化。用Dil标记胎鼠腭架，并使其在保持在器官培养系统中的同时进行融合。在腭融合的几个阶段的组织中检查Dil的分布、特异性抗原的存在和细胞的超微结构外观。Dil标记的腭突在腭融合的各个阶段均占据中线位置。最初的细胞有角蛋白中间丝，并从下面的间充质分离的完整的基底膜。在融合过程中，基底膜降解，Dil标记的细胞与间充质来源的细胞外基质接触。在融合的后期阶段，Dil标记的血管改变了它们的细胞形态，有波形蛋白中间丝，并且与可识别的基底膜无关。Dil标记的细胞，没有上皮表型，仍然存在于完全融合的腭的中线。这些数据表明，腭突没有死亡，但经历了一个表型转化为活的间充质细胞类型，这是保留在腭间充质。
The fate of the medial edge epithelial (MEE) cells during palatal fusion has been proposed to be either programmed cell death or epithelial-mesenchymal transformation. Vital cell labeling techniques were used to mark the MEE and observe their fate during palatal fusion in vitro. Fetal mouse palatal shelves were labeled with Dil and allowed to proceed through fusion while maintained in an organ culture system. The tissues were examined at several stages of palatal fusion for the distribution of Dil, presence of specific antigens and ultrastructural appearance of the cells. The MEE labeled with Dil occupied a midline position at all stages of palatal fusion. Initially the cells had keratin intermediate filaments and were separated from the underlying mesenchyme by an intact basement membrane. During the process of fusion the basement membrane was degraded and the Dil-labeled MEE were in contact with the mesenchymal-derived extracellular matrix. In the late stages of fusion the Dil-labeled MEE altered their cellular morphology, had vimentin intermediate filaments, and were not associated with an identifiable basement membrane. Dil-labeled cells, without an epithelial phenotype, remained present in the midline of the completely fused palate. The data indicate that the MEE did not die but underwent a phenotypic transformation to viable mesenchymal cell types, which were retained in the palatal mesenchyme.