CYTOKINE MESSENGER-RNA PROFILES IN INFLAMMATORY BOWEL-DISEASE MUCOSA DETECTED BY POLYMERASE CHAIN-REACTION AMPLIFICATION
CYTOKINE MESSENGER-RNA PROFILES IN INFLAMMATORY BOWEL-DISEASE MUCOSA DETECTED BY POLYMERASE CHAIN-REACTION AMPLIFICATION
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DOI：
10.1016/0016-5085(92)91182-4
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发表时间：
1992-11-01
期刊：
影响因子：
29.4
通讯作者：
HASKILL, S
中科院分区：
文献类型：
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作者：
ISAACS, KL;SARTOR, RB;HASKILL, S
细胞因子的免疫调节特性可能介导溃疡性结肠炎（UC）和克罗恩病（CD）中的紊乱性炎症事件。在本研究中，在43例患有和不患有炎症性肠病（IBD）的患者的结肠组织中研究了由活化的巨噬细胞产生的细胞因子的概况。采用聚合酶链反应技术研究了从粘膜活检标本中提取的细胞因子信使RNA（mRNA）。活动性UC样本中白细胞介素（IL）1、6和8 mRNA的可检测水平高于非活动性UC和非炎症对照样本。这些细胞因子在活动性UC和炎症对照中相当。活动期UC组织中gromRNA的表达明显高于活动期CD组织。肿瘤坏死因子仅在43个样本中的7个中检测到，组间无差异。活动和非活动CD细胞因子mRNA表达的百分比没有差异。IL-1受体拮抗剂（IL-1 ra）在炎症对照中的表达多于CD，在IBD患者中的表达少于IL-1。严重失活CD中促炎细胞因子的表达和可能的IL-1 ra产生缺陷可以解释疾病的再激活和慢性化。
Immunoregulatory properties of cytokines may mediate disordered inflammatory events in ulcerative colitis (UC) and Crohn's disease (CD). In the present study, profiles of cytokines produced by activated macrophages were studied in colonic tissue from 43 patients with and without inflammatory bowel disease (IBD). Cytokine messenger RNA (mRNA) extracted from mucosal biopsy specimens was studied using polymerase chain reaction assay techniques. A greater percentage of active UC samples had detectable levels of mRNA for interleukins (IL) 1, 6, and 8 andgrothan samples in inactive UC and noninflammatory controls. These cytokines were comparable in active UC and inflammatory controls. Expression ofgromRNA in active UC tissue was significantly higher than in active CD. Tumor necrosis factor was detected in only 7 of 43 samples with no difference between groups. Active and inactive CD did not differ in percentage of cytokine mRNA expression. IL-1 receptor antagonist (IL-1ra) was detected in more inflammatory controls than in CD and was expressed in fewer IBD patients than IL-1. Expression of proinflammatory cytokines in grossly inactive CD and possible defective production of IL-1ra may explain disease reactivation and chronicity.