DDX4 (DEAD box polypeptide 4) colocalizes with cancer stem cell marker CD133 in ovarian cancers
DDX4 (DEAD box polypeptide 4) colocalizes with cancer stem cell marker CD133 in ovarian cancers
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DOI：
10.1016/j.bbrc.2014.03.144
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发表时间：
2014-05-02
影响因子：
3.1
通讯作者：
Cha, Hee-Jae
中科院分区：
文献类型：
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作者：
Kim, Ki Hyung;Kang, Yun-Jeong;Cha, Hee-Jae
DDX 4（DEAD box polypeptide 4，DEAD box 4）是一种RNA解旋酶，具有Asp-Glu-Ala-Asp（DEAD）保守基序，参与RNA二级结构的改变，如翻译起始、核和线粒体剪接、核糖体和剪接体组装等。已知DDX 4是生殖细胞特异性蛋白质，并且用作生殖系干细胞的分选标记物以用于产生卵母细胞。最近关于DDX 4在卵巢癌中的研究表明，DDX 4在上皮性卵巢癌中过表达，并破坏DNA损伤诱导的G2检查点，我们通过组织芯片分析DDX 4和肿瘤干细胞标志物CD 133在卵巢癌中的表达模式，研究DDX 4与卵巢癌干细胞的关系。DDX 4和CD 133在卵巢癌中的表达均显著高于良性肿瘤，且表达模式相似。此外，DDX 4和CD 133大多在各种类型的卵巢癌组织中共定位。此外，几乎所有CD 133阳性卵巢癌细胞也表达DDX 4，而CD 133阴性细胞不具有DDX 4，这表明DDX 4在癌症干细胞中起重要作用的可能性很大，和/或可以用作卵巢癌干细胞标记物。(C)2014爱思唯尔公司All rights reserved.
DDX4 (DEAD box polypeptide 4), characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is an RNA helicase which is implicated in various cellular processes involving the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DDX4 is known to be a germ cell-specific protein and is used as a sorting marker of germline stem cells for the production of oocytes. A recent report about DDX4 in ovarian cancer showed that DDX4 is overexpressed in epithelial ovarian cancer and disrupts a DNA damage-induced G2 checkpoint.We investigated the relationship between DDX4 and ovarian cancer stem cells by analyzing the expression patterns of DDX4 and the cancer stem cell marker CD133 in ovarian cancers via tissue microarray. Both DDX4 and CD133 were significantly increased in ovarian cancer compared to benign tumors, and showed similar patterns of expression. In addition, DDX4 and CD133 were mostly colocalized in various types of ovarian cancer tissues. Furthermore, almost all CD133 positive ovarian cancer cells also express DDX4 whereas CD133-negative cells did not possess DDX4, suggesting a strong possibility that DDX4 plays an important role in cancer stem cells, and/or can be used as an ovarian cancer stem cell marker. (C) 2014 Elsevier Inc. All rights reserved.