Differential Immune Activation During Simulated Cardiopulmonary Bypass Procedure Using Freshly Drawn and Week-Old Blood-A Pilot Study
Differential Immune Activation During Simulated Cardiopulmonary Bypass Procedure Using Freshly Drawn and Week-Old Blood-A Pilot Study
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DOI：
10.1111/j.1525-1594.2010.01122.x
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发表时间：
2010-11-01
影响因子：
2.4
通讯作者：
Uendar, Akif
中科院分区：
文献类型：
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作者：
Aran, Kiana;Fok, Alex;Uendar, Akif
本研究的目的是评估在模拟Penn State Hershey儿科心肺转流（CPB）模型期间使用不同老化血液是否影响免疫激活。为了研究和比较模拟CPB期间体液免疫应答中涉及的细胞因子释放，在相同灌注条件下，在模拟CPB回路中循环献血后不到1小时使用的新鲜抽取的全血（n = 2）和重建的全血（1周龄）（n = 3）。在两个实验中收集离散样本，并使用免疫荧光细胞计数法作为免疫激活的指标分析促炎细胞因子肿瘤坏死因子-α、白细胞介素（IL）-6和IL-8的浓度。结果表明，在CPB循环时间内，新鲜抽取血液的细胞因子浓度与重构血液相比显著增加。对于所分析的所有细胞因子，新鲜血液活化比周龄血液大两到三个数量级。这些结果表明，需要使用新鲜抽取的血液来评价对体外循环的免疫反应。
The goal of this study is to assess whether the use of different aged blood, used during the simulated Penn State Hershey Pediatric cardiopulmonary bypass (CPB) model affects immune activation. In order to study and compare the cytokine release involved in the humoral immune response during simulated CPB, both freshly drawn whole blood used less than 1 h after donation (n = 2) and reconstituted whole blood (1 week old) (n = 3) were circulated in a simulated CPB circuit under identical perfusion conditions. Discrete samples were collected in both experiments and analyzed for the proinflammatory cytokines concentrations of tumor necrosis factor-alpha, interleukin (IL)-6, and IL-8 using immunofluorocytometry as an indicator of immune activation. The results indicated that the cytokine concentrations of freshly drawn blood increased significantly compared with the reconstituted blood over the CPB circulation time. The fresh blood activation was two to three orders of magnitude larger than the week-old blood for all cytokines analyzed. These results suggest that the use of freshly drawn blood is required to evaluate immune responses to the extracorporeal circulation.