Intra-Operative Tissue Oxygen Tension Is Increased by Local Insufflation of Humidified-Warm CO2 during Open Abdominal Surgery in a Rat Model
Intra-Operative Tissue Oxygen Tension Is Increased by Local Insufflation of Humidified-Warm CO2 during Open Abdominal Surgery in a Rat Model
复制标题
DOI：
10.1371/journal.pone.0122838
复制
发表时间：
2015-04-02
期刊：
影响因子：
3.7
通讯作者：
van der Linden, Jan
中科院分区：
文献类型：
--
作者：
Marshall, Jean K.;Lindner, Pernilla;van der Linden, Jan
手术期间建议维持高组织氧合（PtO 2），因为PtO 2高度预测手术部位感染和结肠吻合口瘘。然而，手术部位灌注通常是次优的，为传统的全身应用治疗创造了障碍，以维持或增加手术部位PtO 2。本研究测试的假设，吹入加湿温暖的CO2到腹腔会增加腹膜下PtO 2在开腹surgery.Materials and Methods 15只Wistar大鼠在全身麻醉下进行剖腹手术。进行了三组随机交叉实验，其中腹腔交替暴露于1）加湿-温暖的CO2和环境空气; 2）加湿-温暖的CO2和干燥-寒冷的CO2;和3）干燥-寒冷的CO2和环境空气。结果经湿化温CO2吹入后，PtO 2升高29.8 mmHg（SD 13.3; p
IntroductionMaintenance of high tissue oxygenation (PtO2) is recommended during surgery because PtO2 is highly predictive of surgical site infection and colonic anastomotic leakage. However, surgical site perfusion is often sub-optimal, creating an obstructive hurdle for traditional, systemically applied therapies to maintain or increase surgical site PtO2. This research tested the hypothesis that insufflation of humidified-warm CO2 into the abdominal cavity would increase sub-peritoneal PtO2 during open abdominal surgery.Materials and Methods15 Wistar rats underwent laparotomy under general anesthesia. Three sets of randomized cross-over experiments were conducted in which the abdominal cavity was subjected to alternating exposure to 1) humidified-warm CO2 & ambient air; 2) humidified-warm CO2 & dry-cold CO2; and 3) dry-cold CO2 & ambient air. Sub-peritoneal PtO2 and tissue temperature were measured with a polarographic oxygen probe.ResultsUpon insufflation of humidified-warm CO2, PtO2 increased by 29.8 mmHg (SD 13.3; p