A major and stable QTL associated with seed weight in soybean across multiple environments and genetic backgrounds
A major and stable QTL associated with seed weight in soybean across multiple environments and genetic backgrounds
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DOI：
10.1007/s00122-014-2304-0
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发表时间：
2014-06-01
影响因子：
5.4
通讯作者：
Ishimoto, Masao
中科院分区：
文献类型：
--
作者：
Kato, Shin;Sayama, Takashi;Ishimoto, Masao
利用两个重组自交系群体检测到一个在多个环境和遗传背景下稳定的大豆单粒重QTL。SSW受遗传控制，但这种控制的分子机制仍不清楚。我们现在已经调查了大豆SSW的数量性状位点（QTL），并确定了这样一个QTL，它在多种环境和遗传背景下是稳定的。两个群体的225和250重组自交系的开发，从日本和美国大豆品种之间的杂交，不同的SSW的一个因素相似的2，这些群体生长在至少三个不同的环境。一个全基因组面板包括304个简单重复序列（SSR）位点被应用到每个群体的定位。共检测到15个显著的SSW QTL，分布在两个群体的11条染色体上。在所有环境下生长的两个群体中均检测到位于第17染色体Sat_284和Sat_292之间的一个QTL（3.6 < LOD < 14.1）。该QTL暂命名为qSw 17 -1，占SSW表型变异的9.4- 20.9%，其中一个显性等位基因与SSW增加相关。qSw 17 -1基因对大豆SSW具有显著的影响，是一个有吸引力的定位克隆目标，与该基因座紧密相关的SSR标记可用于大豆SSW控制的标记辅助选择。
We detected a QTL for single seed weight in soybean that was stable across multiple environments and genetic backgrounds with the use of two recombinant inbred line populations.Single seed weight (SSW) in soybean is a key determinant of both seed yield and the quality of soy food products, and it exhibits wide variation. SSW is under genetic control, but the molecular mechanisms of such control remain unclear. We have now investigated quantitative trait loci (QTLs) for SSW in soybean and have identified such a QTL that is stable across multiple environments and genetic backgrounds. Two populations of 225 and 250 recombinant inbred lines were developed from crosses between Japanese and US cultivars of soybean that differ in SSW by a factor of similar to 2, and these populations were grown in at least three different environments. A whole-genome panel comprising 304 simple sequence repeat (SSR) loci was applied to mapping in each population. We identified 15 significant QTLs for SSW dispersed among 11 chromosomes in the two populations. One QTL located between Sat_284 and Sat_292 on chromosome 17 was detected (3.6 < LOD < 14.1) in both populations grown in all environments. This QTL, tentatively designated qSw17-1, accounted for 9.4-20.9 % of phenotypic variation in SSW, with a dominant allele being associated with increased SSW. Given its substantial effect on SSW, qSw17-1 is an attractive target for positional cloning, and SSR markers closely associated with this locus may prove useful for marker-assisted selection for SSW control in soybean.