Auxin-dependent cell expansion mediated by overexpressed auxin-binding protein 1
Auxin-dependent cell expansion mediated by overexpressed auxin-binding protein 1
复制标题
DOI：
10.1126/science.282.5391.1114
复制
发表时间：
1998-11-06
期刊：
影响因子：
56.9
通讯作者：
Binns, AN
中科院分区：
文献类型：
--
作者：
Jones, AM;Im, KH;Binns, AN
为了检验生长素结合蛋白 1 (ABP1) 是控制生长素介导的植物细胞扩张的受体这一假设，ABP1 互补 DNA 在烟草植物和玉米细胞系中以可控方式表达。在烟草叶条中诱导拟南芥ABP1表达导致生长素介导的细胞扩增的能力增加，而在完整植物中诱导ABP1导致叶子具有正常形态，但细胞更大。同样，玉米细胞系中玉米 ABP1 的组成型表达赋予它们通过增加细胞大小来响应生长素的能力。这些结果支持 ABP1 作为生长素受体控制植物生长的作用。
To test the hypothesis that auxin-binding protein 1 (ABP1) is a receptor controlling auxin-mediated plant cell expansion, ABP1 complementary DNAs were expressed in a controllable fashion in tobacco plants and constitutively in maize cell lines. Induction of Arabidopsis ABP1 expression in tobacco leaf strips resulted in an increased capacity for auxin-mediated cell expansion, whereas induction of ABP1 in intact plants resulted in leaves with a normal morphology, but larger cells. Similarly, constitutive expression of maize ABP1 in maize cell lines conferred on them the capacity to respond to auxin by increasing cell size. These results support a role of ABP1 as an auxin receptor controlling plant growth.