Two inactive fragments derived from the yeast mitochondrial ribosomal protein MrpS28 function in trans to support ribosome assembly and respiratory growth.
Two inactive fragments derived from the yeast mitochondrial ribosomal protein MrpS28 function in trans to support ribosome assembly and respiratory growth.
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源自酵母线粒体核糖体蛋白 MrpS28 的两个无活性片段以反式发挥作用，支持核糖体组装和呼吸生长。
DOI：
10.1006/jmbi.1993.1538
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发表时间：
1993
影响因子：
5.6
通讯作者：
Ellis,SR
中科院分区：
文献类型：
--
作者：
Huff,MO;Hanic-Joyce,PJ;Dang,H;Rodrigues,LA;Ellis,SR
酵母线粒体核糖体蛋白MrpS28是与大肠杆菌核糖体蛋白同源的几种线粒体核糖体蛋白之一。相对于与E. coliribosomal蛋白S15是成熟的MrpS28蛋白，其氨基末端和羧基末端分别具有117个和48个氨基酸的独特序列结构域。为了更好地理解MrpS28蛋白的各种序列结构域在体内的作用，在它们是功能性MrpS28蛋白的唯一潜在来源的条件下表达截短的衍生物。本文所示的结果表明，氨基末端结构域和S15样结构域都是呼吸生长所必需的。有趣的是，一个无活性的氨基末端片段可以被包含S15样结构域和羧基末端48个氨基酸的第二个无活性片段反式互补。因此，当这些片段单独或一起表达时，可以检查它们组装成核糖体亚基的情况。从这些研究的结果表明，每个MrpS28衍生的片段促进其他纳入37 S核糖体亚基。
The mitochondrial ribosomal protein MrpS28 ofSaccharomyces cerevisiaeis one of several mitochondrial ribosomal proteins homologous toEscherichia coliribosomal proteins within the context of a larger protein. Relative to a region of homology withE. coliribosomal protein S15, the mature MrpS28 protein has unique sequence domains of 117 and 48 amino acids at its amino and carboxyl terminus, respectively. To better understand the role of the various sequence domains of the MrpS28 proteinin vivo, truncated derivatives were expressed under conditions where they were the only potential source of functional MrpS28 protein. The results shown here demonstrate that the amino-terminal domain and the S15-like domain are both essential for respiratory growth. Interestingly an inactive amino-terminal fragment can be complementedin transby a second inactive fragment comprising the S15-like domain and the carboxyl-terminal 48 amino acids. Consequently, the assembly of these fragments into ribosomal subunits can be examined when they are expressed individually or together. Results from these studies indicate that each of the MrpS28-derived fragments facilitates the incorporation of the other into 37 S ribosomal subunits.