Multiplex measurement of cytokine/receptor gene polymorphisms and interaction between interleukin-10 (-1082) genotype and chorioamnionitis in extreme preterm delivery
Multiplex measurement of cytokine/receptor gene polymorphisms and interaction between interleukin-10 (-1082) genotype and chorioamnionitis in extreme preterm delivery
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DOI：
10.1016/j.jsgi.2006.04.004
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发表时间：
2006-07-01
期刊：
影响因子：
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通讯作者：
Dammann, Olaf
中科院分区：
文献类型：
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作者：
Kerk, Julia;Doerdelmann, Michael;Dammann, Olaf
目的：建立液体和干全血多重扩增难治性突变系统（ARMS），并初步研究炎症相关基因单核苷酸多态性（SNPs）的作用（白细胞介素[IL]-1和-10，肿瘤坏死因子-α [TNFA]和Toll样受体-4 [TLR 4]）及其与早产儿临床绒毛膜炎（CAM）的相互作用。我们建立了一个四重ARMS检测上述四个SNPs。对54名孕龄小于32周的妇女和83名健康女性志愿者进行基因分型。我们比较了（1）早产儿母亲与志愿者，（2）孕29周前分娩的妇女（n = 29）与孕29 ~ 31周分娩的妇女（n = 25）。我们发现早产儿母亲和志愿者的基因型和等位基因频率没有总体差异。在有早产史的妇女中，同时携带CAM和IL 10（-1082）*G等位基因者，在29周前分娩的危险性显著增加（比值比[OR] 22，95%可信区间[CI] 2.5 ~ 191）。结论：CAM和IL 10（-1082）* G等位基因的同时存在可能在小于29周的极端早产中起作用。
OBJECTIVES: To establish a multiplex amplification refractory mutation system (ARMS) in fluid and dried whole blood, and to perform a pilot study to examine the role for single-nucleotide polymorphisms (SNPs) of inflammation-associated genes (interleukin [IL]-1 and -10, tumor necrosis factor-alpha [TNFA], and toll-like receptor-4 [TLR4]) and their interaction with clinical chorioamnionitis (CAM) in prematurity.METHODS: We established a quadruplex ARMS to detect the four above SNPs. Fifty-four women delivered at gestational age less than 32 weeks and 83 healthy female volunteers were genotyped. We compared (1) mothers of preterm infants with volunteers, and (2) women delivered before 29 weeks' gestation (n = 29) with those delivered at 29 to 31 completed weeks (n = 25).RESULTS: Multiplex ARMS is feasible using both fluid and dried whole blood. We found no overall differences in genotype and allele frequencies between mothers of preterm infants and volunteers. Among women who had a preterm delivery, those with both CAM and IL10(- 1082)*G allele, the risk for delivery before 29 weeks was markedly increased (odds ratio [OR] 22, 95% confidence interval [CI] 2.5 - 191).CONCLUSION: The presence of both CAM and 1L10(- 1082)* G might play a role in extreme preterm delivery less than 29 weeks.