DEVELOPMENT OF A DUPLEX PCR ASSAY FOR THE SIMULTANEOUS DETECTION OF FUSARIUM POAE AND FUSARIUM SPOROTRICHIOIDES FROM WHEAT
DEVELOPMENT OF A DUPLEX PCR ASSAY FOR THE SIMULTANEOUS DETECTION OF FUSARIUM POAE AND FUSARIUM SPOROTRICHIOIDES FROM WHEAT
复制标题
开发同时检测小麦中早熟禾镰刀菌和分生孢子镰刀菌的双重 PCR 检测方法
DOI：
10.4454/jpp.v90i3.685
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发表时间：
2008
影响因子：
2.2
通讯作者：
T. Kulik
中科院分区：
文献类型：
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作者：
T. Kulik
镰刀菌属的复合体在全世界的多种宿主植物中引起广泛的疾病。在谷物中，赤霉病是最具破坏性的疾病之一。除产量损失外，镰刀菌对种子的侵染也是一个重要因素。导致各种霉菌毒素在食物中积累。在小粒谷类中，F. poae和F.孢子毛状菌是具有这种致病特性的主要病原体。本研究建立了一种基于多拷贝rDNA序列的高灵敏度二重PCR方法，用于F. poae和F.孢子毛状体在来自世界不同地区和不同宿主或底物的广泛镰刀菌属物种上证明了该测定法的特异性。当与基于单拷贝引物对无菌小麦种子样品的测试相比时，我们的检测被证明具有最高的灵敏度。使用在源自波兰北方的无症状小麦种子样本上开发的标记显示，所有样本均呈F. Poae。
A complex of Fusarium species causes a wide range of diseases in a diversity of host plants worldwide. In cereals Fusarium head blight is one of the most devastating diseases. Besides yield loss, infection of the seeds by Fusarium spp. results in the accumulation of various mycotoxins in food. In small-grain cereals, F. poae and F. sporotrichioides are the main pathogens with such a toxigenic profile. In this study, a highly sensitive duplex PCR assay based on multi-copy rDNA sequences was developed for the differential detection of F. poae and F. sporotrichioides. The assay’s specificity was demonstrated on a wide range of Fusarium species from different parts of the world and from different hosts or substrates. When compared with tests based on single-copy sets of primers on symptomless wheat seed samples, our assay proved to have the highest sensitivity. The use of a marker developed on symptomless wheat seed samples originating from northern Poland showed that all samples were positive for the presence of F. poae.