Erratum: The bacterial DnaA-trio replication origin element specifies single-stranded DNA initiator binding.
Erratum: The bacterial DnaA-trio replication origin element specifies single-stranded DNA initiator binding.
复制标题
勘误表：细菌 DnaA-trio 复制起点元件指定单链 DNA 起始子结合。
DOI：
10.1038/nature18932
复制
发表时间：
2016
期刊：
影响因子：
64.8
通讯作者：
Richardson TT
中科院分区：
文献类型：
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作者：
Richardson TT
DNA复制受到严格控制，以确保遗传信息的准确遗传。在所有生物体中，具有aaa+ (与各种细胞活动相关的ATPase)结构域的启动子蛋白与复制起点结合，以许可新一轮的DNA合成。在细菌中，主启动子蛋白DNAA是高度保守的，具有两个关键的DNA结合活性。Dna A单体通过结合双链DNA序列(Dna A-box)识别复制起点(ORIC)；随后，Dna A细丝通过接合和拉伸单链DNA来组装和促进双链解旋。虽然DNAA对双链DNAA-box的特异性已经被认识了30多年，但单链DNA结合的序列特异性仍然未知。在这里，我们确定了一个新的不可或缺的细菌复制起始元件，由一个重复的三核苷酸基序组成，我们称之为Dna A-trio。我们表明，DNAA-Trio的功能是稳定单链DNA上的DNAA细丝，从而为这种结合机制提供必要的精确度。生物信息学分析在整个细菌界的复制起始点检测到Dna A-trios，表明该元件是核心结构的一部分。新的DNAA-TRIO的发现和鉴定扩展了我们对细菌DNA复制起始的基本理解，并且由于AAA+ 启动子蛋白的保守结构，这些发现增加了在高等生物的复制起源中建立特定识别基序的可能性。
DNA replication is tightly controlled to ensure accurate inheritance of genetic information. In all organisms, initiator proteins possessing AAA+ (ATPases associated with various cellular activities) domains bind replication origins to license new rounds of DNA synthesis. In bacteria the master initiator protein, DnaA, is highly conserved and has two crucial DNA binding activities. DnaA monomers recognize the replication origin (oriC) by binding double-stranded DNA sequences (DnaA-boxes); subsequently, DnaA filaments assemble and promote duplex unwinding by engaging and stretching a single DNA strand,,. While the specificity for duplex DnaA-boxes by DnaA has been appreciated for over 30 years, the sequence specificity for single-strand DNA binding has remained unknown. Here we identify a new indispensable bacterial replication origin element composed of a repeating trinucleotide motif that we term the DnaA-trio. We show that the function of the DnaA-trio is to stabilize DnaA filaments on a single DNA strand, thus providing essential precision to this binding mechanism. Bioinformatic analysis detects DnaA-trios in replication origins throughout the bacterial kingdom, indicating that this element is part of the coreoriCstructure. The discovery and characterization of the novel DnaA-trio extends our fundamental understanding of bacterial DNA replication initiation, and because of the conserved structure of AAA+ initiator proteins these findings raise the possibility of specific recognition motifs within replication origins of higher organisms.