Intracytoplasmic sperm injection (ICSI): the Brussels experience.
Intracytoplasmic sperm injection (ICSI): the Brussels experience.
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胞浆内单精子注射（ICSI）：布鲁塞尔经验。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
S. Silber
中科院分区：
文献类型：
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作者：
H. Tournaye;J. Liu;Z. Nagy;H. Joris;A. Wisanto;M. Bonduelle;der Elst J Van;C. Staessen;J. Smitz;S. Silber
本报告涵盖了对919对夫妇进行1275个连续卵胞浆内单精子注射（ICSI）治疗周期的26个月期间的结果。这些夫妇都受到男性因素不育的困扰，并且至少有过一次失败的常规体外受精（IVF）治疗周期。在其他夫妇中，丈夫的精液参数与常规试管受精不相容或患有排泄性无精子症，这需要显微手术附睾精子抽吸或睾丸精子回收。总的来说，每个取出的卵丘复合体的2原核受精率为47.7%，每个成功注射的中期II卵母细胞的PN受精率为66.4%。90.8%的开始周期进行胚胎移植，362例临床妊娠记录，每个开始周期的临床妊娠率为28.4%，每次移植的临床妊娠率为31.3%。此外，关于微辅助受精后妊娠结局的最新结果也被提出。截至1994年8月30日，已有416名儿童出生。虽然已观察到16例重大先天性畸形（3.9%），但似乎没有理由严重关注ICSI后发生的重大先天性畸形。
The present report covers the results of a 26-month period in which 1275 consecutive treatment cycles by intracytoplasmic sperm injection (ICSI) were performed in 919 couples. These couples were afflicted with male factor infertility and had had at least one previous failed conventional in vitro fertilization (IVF) treatment cycle. In other couples, the husband had semen parameters incompatible with conventional IVF or suffered from excretory azoospermia which required microsurgical epididymal sperm aspiration or testicular sperm retrieval. Overall, the 2 pronuclear (PN) fertilization rate was 47.7% per retrieved oocyte-cumulus complex and 66.4% per successfully injected metaphase II oocyte. Embryo transfer was performed in 90.8% of started cycles and 362 clinical pregnancies were recorded, giving a clinical pregnancy rate of 28.4% per started cycle or 31.3% per transfer. In addition, updated results on the outcome of pregnancies after microassisted fertilization are presented. As of 30 August 1994, 416 children have been born. Although 16 major congenital malformations have been observed (3.9%), there appears to be no reason for serious concern as regards the occurrence of major congenital anomalies after ICSI.