Myometrial prostaglandin E2 synthetic enzyme mRNA expression:: spatial and temporal variations with pregnancy and labour
Myometrial prostaglandin E2 synthetic enzyme mRNA expression:: spatial and temporal variations with pregnancy and labour
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DOI：
10.1093/molehr/gal061
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发表时间：
2006-10-01
影响因子：
4
通讯作者：
Johnson, M. R.
中科院分区：
文献类型：
--
作者：
Sooranna, S. R.;Grigsby, P. L.;Johnson, M. R.
我们已经研究了这一假设，即参与PGE（2）合成的酶在人类子宫中的表达是协调的。我们已经研究了（i）参与PGE（2）合成的酶[磷脂酶（cPLA（2）和sPLA（2））的mRNA表达，前列腺素H合酶（PGHS）-2和PG E脱氢酶（PGES-1和-2）]及其与从经历剖腹产（LSCS）的孕妇获得的子宫肌层样品中的炎性细胞因子表达的关系分娩开始前或分娩开始后或分娩前;（ii）IL-1 β、IL-6、TNF-α、PGE（2）和牵张对PGE（2）酶mRNA表达的影响。我们发现cPLA（2）、sPLA（2）和PGHS-2 mRNA表达在分娩样本中更高; cPLA（2）、sPLA（2）、PGHS-2、PGES-1和PGHS-2 mRNA表达在下段样本中比上段样本中更高;胎龄没有影响。PGHS-2 mRNA水平与PGES-1、cPLA（2）、IL-1 β和IL-8相关; PGES-1 mRNA水平与IL-1 β、IL-8和cPLA相关（2）。在原代培养的子宫肌细胞中，cPLA（2）mRNA表达可被IL-1 β和IL-6增加; PGHS-2 mRNA表达可被IL-1 β、PGE（2）和牵张增加; PGES-1 mRNA表达仅被IL-1 β增加。这些数据表明，分娩与参与PGE（2）合成的酶的表达增加有关，它们在子宫下段的表达更高。PGE（2）酶mRNA表达水平与IL-1 β作用之间存在相关性，表明它们的表达是协调的，IL-1 β是负责任的因子。
We have investigated the hypothesis that the expression of the enzymes involved in PGE(2) synthesis in the human uterus is co-ordinated. We have studied (i) the mRNA expression of the enzymes involved in PGE(2) synthesis [phospholipases (cPLA(2) and sPLA(2)), prostaglandin H synthase (PGHS)-2 and PG E synthases (PGES-1 and -2)] and their relationship to the expression of inflammatory cytokines in samples of myometrium obtained from pregnant women undergoing caesarean section (LSCS) either before or after the onset of labour at or before term; and (ii) the effect of IL-1 beta, IL-6, TNF-alpha, PGE(2) and stretch on PGE(2) enzyme mRNA expression. We found that cPLA(2), sPLA(2) and PGHS-2 mRNA expression were greater in labour samples; cPLA(2), sPLA(2), PGHS-2, PGES-1 and -2 mRNA expression were greater in lower- than upper-segment samples; and there was no effect of gestational age. PGHS-2 mRNA levels correlated with those of PGES-1, cPLA(2), IL-1 beta and IL-8; PGES-1 mRNA levels correlated with those of IL-1 beta, IL-8 and cPLA(2). In primary cultures of uterine myocytes, cPLA(2) mRNA expression was increased by IL-1 beta and IL-6; PGHS-2 mRNA expression was increased by IL-1 beta, PGE(2) and stretch; and PGES-1 mRNA expression was increased by IL-1 beta only. These data show that labour is associated with increased expression of the enzymes involved in PGE(2) synthesis and their expression is greater in the lower uterine segment. The presence of associations between the levels of PGE(2) enzyme mRNA expression and the effects of IL-1 beta suggest that their expression is co-ordinated and that IL-1 beta is the responsible factor.