Strains of CHO-K1 cells resistant to Pseudomonas exotoxin A and cross-resistant to diphtheria toxin and viruses.
Strains of CHO-K1 cells resistant to Pseudomonas exotoxin A and cross-resistant to diphtheria toxin and viruses.
复制标题
CHO-K1 细胞株对假单胞菌外毒素 A 具有抗性，并且对白喉毒素和病毒具有交叉抗性。
DOI：
10.1128/iai.41.3.998-1009.1983
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发表时间：
1983
影响因子：
3.1
通讯作者：
Moehring,TJ
中科院分区：
文献类型：
--
作者：
Moehring,JM;Moehring,TJ
我们已经研究了两种表型不同类型的突变体的CHO-K1细胞，是耐假单胞菌外毒素A由于缺陷的活性毒素在细胞中的靶位点，延伸因子2。这两种类型都含有正常水平的毒素敏感延伸因子-2。杂交研究表明，这些细胞分为两个不同的互补组。一组称为DPVr，对假单胞菌毒素、白喉毒素和四种包膜RNA病毒具有抗性。这个群体对蓖麻毒素也非常敏感。这一群体的抵抗力显然与内吞囊泡酸化机制的缺陷有关。另一组称为PVr，对假单胞菌毒素和三种包膜RNA病毒具有抗性。该组的耐药性似乎与辛德毕斯病毒成熟所需的细胞机制的缺陷有关，辛德毕斯病毒成熟同样是活性假单胞菌毒素进入所需的。
We have investigated two phenotypically distinct types of mutants of CHO-K1 cells that are resistant to Pseudomonas exotoxin A due to a defect in the delivery of active toxin to the target site in the cell, elongation factor 2. Both types contain normal levels of toxin-sensitive elongation factor-2. Hybridization studies have shown that these cells fall into two distinct complementation groups. One group, designated DPVr, is resistant to Pseudomonas toxin, diphtheria toxin, and four enveloped RNA viruses. This group is also hypersensitive to ricin. The resistance of this group is apparently related to a defect in a mechanism for the acidification of endocytic vesicles. The other group, designated PVr, is resistant to Pseudomonas toxin and to three enveloped RNA viruses. The resistance of this group appears to be related to a defect in a cellular mechanism required for the maturation of Sindbis virus that is likewise required for the entry of active Pseudomonas toxin.