Microbial Communities Associated with Potato Common Scab-Suppressive Soil Determined by Pyrosequencing Analyses
Microbial Communities Associated with Potato Common Scab-Suppressive Soil Determined by Pyrosequencing Analyses
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DOI：
10.1094/pdis-07-11-0571
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发表时间：
2012-05-01
期刊：
影响因子：
4.5
通讯作者：
Hao, Jianjun J.
中科院分区：
文献类型：
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作者：
Rosenzweig, Noah;Tiedje, James M.;Hao, Jianjun J.
马铃薯常见的赤霉病是马铃薯种植户每年都会遇到的问题，目前的方法无法有效控制赤霉病的发生。在密歇根州发现了一个自然发生的常见疮痂抑制领域，并证实具有这种疾病抑制的生物学基础。利用焦磷酸测序技术对这片田地和邻近的一个有利于疾病发生的疮痂苗圃进行了研究，比较了两种微生物群落。从有益疾病和抑制疾病的土壤中提取总DNA。利用16S rRNA基因的系统发育分类信息区建立操作分类单位（OTUs），以表征细菌群落的丰富度和多样性。共检测到1124个otu，在有益土壤中鉴定出565个otu（差异10%），在抑制土壤中鉴定出859个otu，其中300个otu在两个站点之间共有。基于相对序列丰度的常见门是酸杆菌门、变形菌门和厚壁菌门。在抑制疾病的土壤中，溶菌菌序列的数量显著增加，第4组和第6组酸杆菌序列的数量也显著增加。在有利于致病的土壤中，鉴定为芽孢杆菌属的序列的相对丰度显著高于一个数量级。
Potato common scab, caused by Streptomyces spp., is an annual production problem for potato growers, and not effectively controlled by current methods. A field with naturally occurring common scab suppression has been identified in Michigan, and confirmed to have a biological basis for this disease suppression. This field and an adjacent scab nursery conducive to disease were studied using pyrosequencing to compare the two microbial communities. Total DNA was extracted from both the disease-conducive and -suppressive soils. A phylogenetically taxon-informative region of the 16S rRNA gene was used to establish operational taxonomic units (OTUs) to characterize bacterial community richness and diversity. In total, 1,124 OTUs were detected and 565 OTUs (10% dissimilarity) were identified in disease-conducive soil and 859 in disease-suppressive soil, including 300 shared both between sites. Common phyla based on relative sequence abundance were Acidobacteria, Proteobacteria, and Firmicutes. Sequences of Lysobacter were found in significantly higher numbers in the disease-suppressive soil, as were sequences of group 4 and group 6 Acidobacteria. The relative abundance of sequences identified as the genus Bacillus was significantly higher by an order of magnitude in the disease-conducive soil.