Evolution of the MHC: antigenicity and unusual tissue distribution of Xenopus (frog) class II molecules.
Evolution of the MHC: antigenicity and unusual tissue distribution of Xenopus (frog) class II molecules.
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MHC 的进化：爪蟾（青蛙）II 类分子的抗原性和异常组织分布。
DOI：
10.1016/0161-5890(90)90170-5
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发表时间：
1990
影响因子：
3.6
通讯作者：
DuPasquier,L
中科院分区：
文献类型：
--
作者：
Flajnik,MF;Ferrone,S;Cohen,N;DuPasquier,L
识别Xenopus II类分子的抗体已经开发出来。用部分纯化的蛙MHC抗原免疫BALB/c小鼠，用活化的爪蟾T淋巴细胞免疫小鼠脾细胞，制备了鼠源性单克隆抗体。此外，还发现5种鼠源性抗人Ⅱ类抗原单克隆抗体与XenopusII类抗原有交叉反应。TH小鼠不表达E II类分子，在用青蛙淋巴细胞免疫后总是产生与青蛙II类分子反应的免疫沉淀抗体;其他小鼠品系很少产生这种抗体。两个单克隆抗体对青蛙的第二类分子识别变性的第二类β链蛋白质印迹，和其他三个似乎只识别第二类异二聚体复合物。抗体显示与爪蟾的等位基因II类产物的差异反应性。单克隆抗体与脾和外周血中的所有成人淋巴细胞、T细胞和B细胞反应，每个细胞具有相等水平的II类抗原。II类分子是成体胸腺细胞上的“分化抗原”，因为在成熟髓质群体上表达最多。在含有胎牛血清的培养基中培养后，II类分子/淋巴细胞的数量增加。顺序免疫沉淀和等电聚焦实验表明，与单克隆抗体免疫沉淀的细胞表面II类分子与先前用于识别青蛙II类分子的DR抗原特异性交叉反应抗血清免疫沉淀的那些分子相同。
Antibodies that recognizeXenopusclass II molecules have been developed. Mouse monoclonal antibodies were prepared by immunizing BALB/c mice with frog MHC antigens that had been partially purified with alloantisera, and by immunizing mouse spleen cellsin vitrowith activated Xenopus T lymphocytes. In addition, five mouse monoclonal antibodies specific for human class II antigens were found to cross-react withXenopusclass II antigens. A.TH mice, which do not express E class II molecules, always produce immunoprecipitating antibodies reactive with frog class II molecules after immunization with frog lymphocytes; other mouse strains rarely produce such antibodies. Two of the monoclonal antibodies raised against frog class II molecules recognize the denatured class II beta chain on Western blots, and the other three appear to recognize only the class II heterodimeric complex. The antibodies display differential reactivity with the allelic class II products ofXenopus. The monoclonal antibodies react with all adult lymphocytes in the spleen and peripheral blood, T cells and B cells having equivalent levels of class II antigens per cell. Class II molecules are “differentiation antigens” on adult thymocytes as the expression is greatest on the mature medullary population. The number of class II molecules/lymphocyte increases after culturing in medium containing fetal bovine serum. Sequential immunoprecipitation and isoelectric focusing experiments have shown that cell surface class II molecules immunoprecipitated with the monoclonal antibodies are the same as those immunoprecipitated with the cross-reactive antiserum specific for DR antigens which was previously used to identify frog class II molecules.