A novel precision-engineered microfiltration device for capture and characterisation of bladder cancer cells in urine.
A novel precision-engineered microfiltration device for capture and characterisation of bladder cancer cells in urine.
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DOI：
10.1016/j.ejca.2013.04.033
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Cote RJ
中科院分区：
文献类型：
--
作者：
Birkhahn M;Mitra AP;Williams AJ;Barr NJ;Skinner EC;Stein JP;Skinner DG;Tai YC;Datar RH;Cote RJ
Sensitivity of standard urine cytology for detecting urothelial carcinoma of the bladder (UCB) is low, attributable largely to its inability to process entire samples, paucicellularity, and presence of background cells. Evaluate performance and practical applicability of a novel portable microfiltration device for capture, enumeration, and characterization of exfoliated tumor cells in urine, and compare it with standard urine cytology for UCB detection. A total of 54 urine and bladder wash samples from patients undergoing surveillance for UCB were prospectively evaluated by standard and microfilter-based urine cytology. Head-to-head comparison of quality and performance metrics, and cost effectiveness was conducted for both methodologies. Five samples were paucicellular by standard cytology; no samples processed by microfilter cytology were paucicellular. Standard cytology had 33.3% more samples with background cells that limited evaluation (p<0.001). Microfilter cytology was more concordant (κ=50.4%) than standard cytology (κ=33.5%) with true UCB diagnosis. Sensitivity, specificity, and accuracy were higher for microfilter cytology compared to standard cytology (53.3%/100%/79.2%, versus 40%/95.8%/69.9%, respectively). Microfilter-captured cells were amenable to downstream on-chip molecular analyses. A 40ml sample was processed in under 4 minutes by microfilter cytology compared to 5.5 minutes by standard cytology. Median microfilter cytology processing and set-up costs were approximately 63% cheaper and 80 times lower than standard cytology, respectively. The microfiltration device represents a novel non-invasive UCB detection system that is economical, rapid, versatile, and has potentially better quality and performance metrics than routine urine cytology, the current standard-of-care.
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影响因子：
45.3
作者：
Mitra, Anirban P.;Pagliarulo, Vincenzo;Cote, Richard J.
通讯作者：
Cote, Richard J.
DOI：
10.1158/1078-0432.ccr-10-1105
发表时间：
2010-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Lin HK;Zheng S;Williams AJ;Balic M;Groshen S;Scher HI;Fleisher M;Stadler W;Datar RH;Tai YC;Cote RJ
通讯作者：
Cote RJ
影响因子：
3.8
作者：
Mitra AP;Almal AA;George B;Fry DW;Lenehan PF;Pagliarulo V;Cote RJ;Datar RH;Worzel WP
通讯作者：
Worzel WP
影响因子：
23.4
作者：
Babjuk, Marko;Oosterlinck, Willem;Roupret, Morgan
通讯作者：
Roupret, Morgan
影响因子：
1.9
作者：
LANDIS, JR;KOCH, GG
通讯作者：
KOCH, GG