Ionization of unconjugated, glycine- and taurine-conjugated bile acids by electrospray ionization mass spectrometry
Ionization of unconjugated, glycine- and taurine-conjugated bile acids by electrospray ionization mass spectrometry
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DOI：
10.1016/j.jpba.2005.09.012
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发表时间：
2006-03-18
影响因子：
3.4
通讯作者：
Goto, J
中科院分区：
文献类型：
--
作者：
Mano, N;Mori, M;Goto, J
研究了有机阴离子作为喷雾液体添加剂对电喷雾电离条件下非共轭胆汁酸、甘氨酸共轭胆汁酸和牛磺酸共轭胆汁酸电离效率的影响。有机酸的加入影响了整个胆汁酸的电离效率。使用更强的酸降低了未共轭和甘氨酸共轭胆汁酸的峰值强度，而使用TFA（测试中最强的酸）提高了牛磺酸缀合物的强度。胆酸和去氧胆酸C-12 α位置的羟基容易与侧链羧基发生分子内氢键，加快了电离效率。这种分子内氢键也可能影响低能cid产物离子的形成。喷淋液中铵离子的加入影响了所有胆汁酸的电离，特别是提高了未共轭胆汁酸的电离效率。(c) 2005 Elsevier B.V.版权所有
We investigated the effect of organic anions as spray liquid additives on the ionization efficiency of unconjugated, glycine-conjugated and taurine-conjugated bile acids under electrospray ionization conditions. Addition of organic acids influenced the ionization efficiency of whole bile acids. Use of a stronger acid reduced the peak intensity of unconjugated and glycine-conjugated bile acids, while the use of TFA, the strongest acid tested, improved the intensity of taurine conjugates. The hydroxyl group at the C-12 alpha position of cholic acid and deoxycholic acid easily underwent intra-molecular hydrogen bonding with the side chain carboxyl group, accelerating the ionization efficiency. This intra-molecular hydrogen bond may also affect the formation of product ions in low energy-CID. The addition of ammonium ions to the spray liquid influenced the ionization of all bile acids, specifically enhancing the ionization efficiency of Unconjugated bile acids. (c) 2005 Elsevier B.V. All rights reserved.