A Revised Iron Extraction Protocol for Environmental Samples Rich in Nitrite and Carbonate
A Revised Iron Extraction Protocol for Environmental Samples Rich in Nitrite and Carbonate
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DOI：
10.1080/01490451.2017.1303554
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发表时间：
2018-01
影响因子：
2.3
通讯作者：
F. Schaedler;A. Kappler;C. Schmidt
中科院分区：
文献类型：
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作者：
F. Schaedler;A. Kappler;C. Schmidt
摘要湿化学提铁法被广泛应用于沉积物和土壤中矿物结合态含铁组分的定量分析。如前所述，这种方法受到土壤/沉积物成分的强烈影响。富含主要由微生物产生的亚硝酸盐的样品需要去除含亚硝酸盐的水相或用氨基磺酸（SA）代替HCl作为萃取剂。在这项研究中，我们表明，沉积碳酸盐缓冲SA，抑制稳定的Fe（II）和铁的有效提取。因此，我们提供了一个修订的提取协议，允许保存低pH值条件，导致有效的铁提取亚硝酸盐和碳酸盐富集的样品。
ABSTRACT Wet-chemical iron extraction is widely applied to quantify the mineral-bound ferriferous fraction of sediments and soils. As previously shown, this method is strongly affected by the composition of the soil/sediment. Samples enriched in mostly microbially produced nitrite require the removal of the nitrite-containing aqueous phase or the replacement of HCl with sulfamic acid (SA) as the extractant. In this study, we show that sedimentary carbonate buffers SA, inhibiting the stabilization of Fe(II) and effective extraction of iron. We therefore provide a revised extraction protocol which allows the preservation of low pH conditions, leading to efficient iron extraction in nitrite- and carbonate-enriched samples.