Vegetation Restoration Alters the Diversity and Community Composition of Soil Nitrogen-Fixing Microorganisms in the Loess Hilly Region of China
Vegetation Restoration Alters the Diversity and Community Composition of Soil Nitrogen-Fixing Microorganisms in the Loess Hilly Region of China
复制标题
植被恢复改变了中国黄土丘陵区土壤固氮微生物的多样性和群落组成
DOI：
10.2136/sssaj2019.03.0066
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发表时间：
2019-09
影响因子：
2.9
通讯作者：
Wenjing Qiao
中科院分区：
文献类型：
--
作者：
Yadong Xu;Wei Zhang;Zekun Zhong;Shujuan Guo;Xinhui Han;Gaihe Yang;Chengjie Ren;Zhengxing Chen;Yinyue Dai;Wenjing Qiao
在这项研究中，我们确定了在造林过程中固氮微生物的多样性和结构的变化，以及这种变化如何与土壤理化因子。土壤取样重复样地代表三种植被类型（刺槐L。[Rp]、柠条锦鸡儿（Caragana korshinskii Kom [Ck]）和撂荒地）与相邻农田作为对照。利用nifH rRNA基因的高通量测序结果发现，植被恢复显著提高了Shannon多样性指数，其中Chao 1的丰富度指数在Ck最高，在其他3个土地上差异不显著。从农田到植被类型、弃耕地、Ck和Rp，nifH微生物的优势门由放线菌门转变为变形菌门。pH（p = 0.002）、土壤有机碳（SOC; p = 0.025）和全氮（TN; p = 0.015）对Chao 1丰富度指数的影响显著。土壤有机碳、硝态氮、C/P、TN、AN、N/P、TP和砂粒对Shannon多样性指数有显著影响。nifH微生物组成在门水平上的变化与土壤氮磷比呈显著正相关，可解释环境因子间变异的53.1%（F = 11.8，p = 0.002）。N/P比值（解释率65.2%，F = 18.8，p = 0.002）也是影响属水平组成的关键因子。从植物的多样性和组成来看，CK是一种更有利于提高固氮能力的恢复方式。本研究为黄土丘陵区土壤固氮特性的研究提供了依据。
In this study, we determined the variations in the diversity and structure of nitrogen-fixing microorganisms during afforestation and how this variability correlates with the soil physicochemical factors. Soils were sampled from replicated plots representing three revegetation types (Robinia pseudoacacia L. [Rp], Caragana korshinskii Kom [Ck], and abandoned land) with adjacent farmland as the control at a research site in Ansai District, Yan’an City, Shaanxi Province, China. Using high-throughput sequencing of the nifH rRNA gene, we found that revegetation significantly increased the Shannon diversity index, and for the richness index Chao1 was highest in Ck and have no significant difference in other three lands. From farmland to vegetation types abandoned land, Ck, and Rp, the dominant phylum of nifH microorganisms changed from Actinobacteria to Proteobacteria. Furthormore, variation in the Chao1 richness index was significantly influenced by pH (p = 0.002), soil organic carbon (SOC; p = 0.025) and total nitrogen (TN; p = 0.015). The Shannon diversity index was significantly influenced by SOC, nitrate nitrogen (NN), C to P ratio, TN, AN, N to P ratio, TP, and sand. The changes of nifH microorganism composition at the phylum level were significantly correlated with the soil N to P ratio, which explained 53.1% of the variation among environmental factors (F = 11.8, p = 0.002). The ratio of N to P (interpretation rate 65.2%, F = 18.8, p = 0.002) was also a key factor that influenced the composition at the genus level. Based on the diversity and composition, Ck was a more advantageous recovery method to increase the capacity for N fixation. This study provides a basis for the study of N-fixation characteristics in the Loess Hilly Region.
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影响因子：
4
作者：
Olander, LP;Vitousek, PM
通讯作者：
Vitousek, PM
DOI：
--
发表时间：
2011
期刊：
Bulletin of Soil and Water Conservation
影响因子：
--
作者：
Lu Qin-an
通讯作者：
Lu Qin-an
影响因子：
3.7
作者：
C. Ren;F. Zhao;Di Kang;Gaihe Yang;Xinhui Han;X. Tong;Yongzhong Feng;Guangxin Ren
通讯作者：
C. Ren;F. Zhao;Di Kang;Gaihe Yang;Xinhui Han;X. Tong;Yongzhong Feng;Guangxin Ren
影响因子：
4.8
作者：
Yuqian Tang;Guirui Yu;Xinyu Zhang;Qiufeng Wang;J. Ge;Shuang-shuang Liu
通讯作者：
Yuqian Tang;Guirui Yu;Xinyu Zhang;Qiufeng Wang;J. Ge;Shuang-shuang Liu
影响因子：
9.7
作者：
Fontaine, S.;Henault, C.;Maron, P. A.
通讯作者：
Maron, P. A.