REGULATORY ELEMENTS WITHIN THE MURINE LEUKEMIA-VIRUS ENHANCER REGIONS MEDIATE GLUCOCORTICOID RESPONSIVENESS
REGULATORY ELEMENTS WITHIN THE MURINE LEUKEMIA-VIRUS ENHANCER REGIONS MEDIATE GLUCOCORTICOID RESPONSIVENESS
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DOI：
10.1128/jvi.62.4.1314-1322.1988
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发表时间：
1988-04-01
影响因子：
5.4
通讯作者：
HASELTINE, WA
中科院分区：
文献类型：
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作者：
CELANDER, D;HSU, BL;HASELTINE, WA
非白血病 (Akv) 和 T 细胞白血病 (SL3-3) 鼠白血病病毒中的增强子元件在转录活性中表现出强烈的细胞类型偏好。这些转录元件还受到合成糖皮质激素地塞米松的调节，并且这种调节模式根据细胞类型而变化。 Akv 和 SL3-3 的地塞米松调节所需的序列包括一个 17 核苷酸共有序列，以前称为糖皮质激素反应元件 (GRE)。尽管两种病毒增强子的 GRE 是相同的，但这些元件周围的序列不同，GRE 序列相对于彼此的空间排列也不同。有人提出，GRE 的空间排列及其精确的序列背景决定了不同细胞类型中增强子对地塞米松反应的差异。
Enhancer elements within nonleukemogenic (Akv) and T-cell leukemogenic (SL3-3) murine leukemia viruses demonstrate strong cell type preference in transcriptional activity. These transcription elements are additionally regulated by the synthetic glucocorticoid dexamethasone, and this pattern of regulation varies according to cell type. The sequences required for dexamethasone regulation for both Akv and SL3-3 are shown to include a 17-nucleotide consensus sequence previously termed the glucocorticoid response element (GRE). Although the GREs are identical for both viral enhancers, the sequences surrounding these elements differ, as does the spatial arrangement of the GRE sequences with respect to one another. It is proposed that the spatial arrangement of the GREs, as well as their precise sequence context, determines the difference in the response to dexamethasone of the enhancers in different cell types.