Regulation of macrophage inflammatory protein-1α expression and function by endogenous interleukin-10 in a model of acute inflammation
Regulation of macrophage inflammatory protein-1α expression and function by endogenous interleukin-10 in a model of acute inflammation
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DOI：
10.1006/bbrc.1999.0196
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发表时间：
1999-02-16
影响因子：
3.1
通讯作者：
Perretti, M
中科院分区：
文献类型：
--
作者：
Ajuebor, MN;Das, AM;Perretti, M
在这项研究中，我们已经确定了内源性白细胞介素（IL）-10的白细胞募集和CC趋化因子巨噬细胞炎性蛋白-1 α（MIP-1 α）在急性炎症小鼠模型的生产的作用。腹膜内注射酵母多糖产生剂量依赖性细胞浸润，其伴随着灌洗液中MIP-1 α的释放。这种趋化因子的释放具有功能性作用，因为用特异性抗MIP-1 α抗体处理小鼠减少了中性粒细胞和单核细胞积聚到腹膜腔中。在耗尽驻留的腹膜巨噬细胞后，测量到细胞流入和MIP-1 α产生的意外增加，如通过3天脂质体处理实现的。当在IL-10基因敲除小鼠中引发酵母多糖腹膜炎反应时，获得了类似的结果。总之，我们提出了在宿主炎症反应过程中内源性IL-10和CC趋化因子之间的功能性串扰。(C)北京：科学出版社.
In this study we have determined the role of endogenous interleukin (IL)-10 on leucocyte recruitment and production of the CC chemokine macrophage inflammatory protein-1 alpha (MIP-1 alpha) in a murine model of acute inflammation. Intraperitoneal injection of zymosan produced a dose-dependent cellular infiltration which was concomitant with MIP-1 alpha release in the lavage fluids. Release of this chemokine had a functional role since treatment of mice with a specific anti-MIP-1 alpha antibody reduced both neutrophil and monocyte accumulation into the peritoneal cavity, An unexpected increase in cell influx and MIP-1 alpha production was measured following depletion of resident peritoneal macrophages, as achieved by a 3-day liposome treatment. A similar result was obtained when the zymosan peritonitis response was elicited in IL-IO knock-out mice. In summary we propose a functional cross talk between endogenous IL-10 and this CC chemokine during the host inflammatory response. (C) 1999 Academic Press.