EFFECTS OF CHALLENGE DOSE ON THE CLINICAL AND IMMUNE-RESPONSES OF CATTLE VACCINATED WITH REDUCED DOSES OF BRUCELLA-ABORTUS STRAIN-19
EFFECTS OF CHALLENGE DOSE ON THE CLINICAL AND IMMUNE-RESPONSES OF CATTLE VACCINATED WITH REDUCED DOSES OF BRUCELLA-ABORTUS STRAIN-19
复制标题
DOI：
10.1016/0378-1135(85)90065-3
复制
发表时间：
1985-12-01
影响因子：
3.3
通讯作者：
SMITH, RA
中科院分区：
文献类型：
--
作者：
CONFER, AW;HALL, SM;SMITH, RA
对57头未接种或既往接种过流产布鲁氏菌S19菌落形成单位(CFU)的妊娠小母牛，结膜内注射强毒流产杆菌S2308，每次9.4倍。106CFU(实验1)或5.2倍。107个CFU(实验2)。在实验1中，S19对9头未接种疫苗的小母牛中的8头、接种了109CFU的11头母牛中的1头和接种了1010 CFU的10头母牛中的3头流产或产下虚弱、不能存活的小牛，提供了显著的保护(P<；0.01)。在实验2中，接种疫苗没有提供显著的保护作用(P>；0.05)，9头未接种疫苗的母牛中有9头流产，10头接种了109CFU的母牛中有8头流产，8头接种了1010CFU的母牛流产。通过三种标准试验以及定量荧光免疫分析(FIAX)和酶联免疫吸附试验来确定对流产杆菌的血清学反应。在实验1中，对照组在攻击后0-8周的早期血清学反应似乎比疫苗接种组大，但在实验2中，疫苗接种组在挑战暴露后0-6周的早期反应似乎比对照组大。以热灭活流产杆菌为抗原，攻击暴露后依次进行淋巴细胞转化试验。一般来说，接种疫苗的小母牛的平均应答显著高于未接种的小母牛(P<；0.05)。对于个体小母牛，淋巴细胞转化试验和临床对挑战暴露的反应之间不能建立联系。
Fifty-seven pregnant beef heifers that were unvaccinated or previously vaccinated with Brucella abortus S19, at a dose of either 109 or 1010 colony-forming units (CFU), were challenge-exposed intraconjunctivally with virulent B. abortus S2308 at a dose of 9.4 .times. 106 CFU (Experiment 1) or 5.2 .times. 107 CFU (Experiment 2). In Experiment 1, S19 afforded significant protection (P < 0.01) against challenge exposure in that 8 of 9 unvaccinated heifers, 1 of 11 vaccinated with 109 CFU and 3 of 10 vaccinated with 1010 CFU aborted or delivered weak, non-viable calves. In Experiment 2, vaccination did not afford significant protection (P > 0.05) in that 9 of 9 unvaccinated heifers, 8 of 10 vaccinated with 109 CFU, and 8 of 8 vaccinated with 1010 CFU aborted. Serologic responses to B. abortus were determined by three standard tests, as well as a quantitative fluorometric immunoassay (FIAX) and an enzyme-linked immunosorbent assay. In Experiment 1, the early serologic response, 0-8 weeks after challenge, appeared greater for controls than for vaccinates, but in Experiment 2, the early response, 0-6 weeks after challenge exposure, appeared greater for vaccinates than for controls. The lymphocyte blast transformation assay, using heat-killed B. abortus as an antigen, was performed sequentially after challenge exposure. In general, mean responses were significantly higher (P < 0.05) for vaccinated than for non-vaccinated heifers. For individual heifers, an association could not be established between the lymphocyte blast transformation assay and the clinical response to challenge exposure.