The Differential Effects of Anesthetics on Bacterial Behaviors.
The Differential Effects of Anesthetics on Bacterial Behaviors.
复制标题
麻醉药对细菌行为的不同影响。
DOI：
10.1371/journal.pone.0170089
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Yuki K
中科院分区：
文献类型：
--
作者：
Chamberlain M;Koutsogiannaki S;Schaefers M;Babazada H;Liu R;Yuki K
挥发性麻醉剂已经在临床上使用了很长一段时间，并且被认为是混杂的，可能与中枢神经系统中的几个离子通道相互作用以产生麻醉。由于离子通道及其现有的进化类似物离子转运蛋白在各种生物体中非常重要，因此挥发性麻醉剂可能会影响某些细菌。在这项研究中，我们假设挥发性麻醉剂可以影响细菌的行为。我们评估了麻醉剂对细菌生长，运动（游泳和滑行）和生物膜形成的四种常见的细菌病原体在体外的影响。我们发现，常用的挥发性麻醉药异氟烷和七氟烷影响细菌的运动性和生物膜的形成没有任何影响的常见细菌病原体的生长在这里研究。利用现有的大肠杆菌离子转运蛋白基因缺失突变体和计算机分子对接，我们认为这些改变的行为可能至少部分是通过挥发性麻醉剂与离子转运蛋白的相互作用。
Volatile anesthetics have been in clinical use for a long period of time and are considered to be promiscuous by presumably interacting with several ion channels in the central nervous system to produce anesthesia. Because ion channels and their existing evolutionary analogues, ion transporters, are very important in various organisms, it is possible that volatile anesthetics may affect some bacteria. In this study, we hypothesized that volatile anesthetics could affect bacterial behaviors. We evaluated the impact of anesthetics on bacterial growth, motility (swimming and gliding) and biofilm formation of four common bacterial pathogens in vitro. We found that commonly used volatile anesthetics isoflurane and sevoflurane affected bacterial motility and biofilm formation without any effect on growth of the common bacterial pathogens studied here. Using available Escherichia coli gene deletion mutants of ion transporters and in silico molecular docking, we suggested that these altered behaviors might be at least partly via the interaction of volatile anesthetics with ion transporters.