Somatodendritic dopamine release in rat substantia nigra influences motor performance on the accelerating rod
Somatodendritic dopamine release in rat substantia nigra influences motor performance on the accelerating rod
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DOI：
10.1016/s0006-8993(03)02555-1
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发表时间：
2003-05-23
期刊：
影响因子：
2.9
通讯作者：
Nissbrandt, H
中科院分区：
文献类型：
--
作者：
Bergquist, F;Shahabi, HN;Nissbrandt, H
采用双探针微透析和同步加速棒运动性能测试相结合的方法，对大鼠黑质体树突多巴胺释放的生理作用进行了评价。三个主要的研究结果支持调节影响体树突多巴胺释放运动协调。(1)视杆细胞性能测试与黑质细胞外多巴胺浓度增加相关，但与5-羟色胺浓度无关，纹状体多巴胺和5-羟色胺浓度均增加。(2)没有内在活性的多巴胺拮抗剂的黑质应用导致加速杆上的性能改变。对黑质灌注低浓度（0.1，1.0 μ M）的D-2/D-3拮抗剂雷氯必利的反应包括杆性能的损害，其为灌注前性能的63%。然而，较高的浓度（10，100 μ M）与受损的杆性能无关，但与纹状体多巴胺浓度增加。用1、10和100 μ M D-1/D-5拮抗剂SCH 23390灌注黑质可剂量依赖性地损害视杆性能。SCH 23390持续增加黑质中的多巴胺和5-羟色胺浓度，但未改变纹状体中的透析液。(3)在单侧6-羟基多巴胺损伤的大鼠中，在用非选择性多巴胺激动剂阿扑吗啡灌注黑质期间观察到杆性能的剂量依赖性改善。(C)2003 Elsevier Science B. V.保留所有权利。
The physiological role of somatodendritic dopamine release in the rat substantia nigra was evaluated with a combination of dual probe microdialysis and simultaneous motor performance tests on an accelerating rod. Three main findings support a modulating influence of somatodendritic dopamine release on motor coordination. (1) The rod performance tests were associated with an increase in extracellular dopamine but not 5-hydroxytryptamine concentrations in substantia nigra and wit h increases in both dopamine and 5-hydroxytryptamine concentrations in the striatum. (2) Nigral application of dopamine antagonists without intrinsic activity resulted in changed performances on the accelerating rod. The response to nigral perfusion with low concentrations (0.1, 1.0 muM) of the D-2/D-3-antagonist raclopride consisted of an impairment in rod performance to 63% of the pre-perfusion performance. Higher concentrations (10, 100 muM), however, were not associated with impaired rod performance, but with increased striatal dopamine concentrations. Perfusion of the substantia nigra with 1, 10 and 100 muM of the D-1/D-5-antagonist SCH 23390 dose-dependently impaired rod performance. SCH 23390 consistently increased dopamine and 5-hydroxytryptamine concentrations in substantia nigra but did not change the dialysate in the striatum. (3) In unilaterally 6-hydroxydopamine-lesioned rats, a dose-dependent improvement in rod performance was observed during perfusion of the substantia nigra with the non-selective dopamine agonist apomorphine. (C) 2003 Elsevier Science B.V. All rights reserved.