Local application of statins in the treatment of experimental periodontal disease in rats.
Local application of statins in the treatment of experimental periodontal disease in rats.
复制标题
DOI：
10.1590/1678-77572016-0149
复制
发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Fernandes LA
中科院分区：
文献类型：
--
作者：
Santos BF;Souza EQ;Brigagão MR;Lima DC;Fernandes LA
本研究的目的是评估他汀类药物作为佐剂在大鼠牙周病刮治和根治（SRP）治疗中的局部作用。本实验用90只大鼠。将棉线置于左第一下颌磨牙处诱发所有动物的牙周病。诱导7 d后，取下绷带，将大鼠分为3组：1)NT组（n=30），不处理；2) SRP组（n=30）： SRP +对照凝胶灌洗；3) S组(n=30) - SRP和辛伐他汀灌洗。各组分别于治疗后7、15、30天对10只动物实施安乐死。处理牙龈活检标本，分析基质金属蛋白酶8 （MMP-8）的表达。下颌骨被移除并提交给x线摄影和实验室处理进行组织计量学分析。与NT和SRP组相比，S组在所有实验期间MMP-8的表达均显著降低。在各组间的影像学和组织计量学分析中，S组在所有实验期间的骨丢失（BL）均明显低于NT组和SRP组。在本研究范围内，可以得出结论，局部应用他汀类药物作为诱导牙周病大鼠SRP的辅助治疗是有效的。
The objective of this study was to evaluate the local effects of statins as adjuvants for treatment by scaling and root planing (SRP) of periodontal disease induced in rats. Ninety rats were used in the present experiment. Periodontal disease was induced in all animals using a cotton thread placed in the left first mandibular molar. After 7 days of induction, the bandage was removed and the animals were divided into three groups: 1) NT group (n=30), no treatment; 2) SRP group (n=30): SRP and irrigation with control gel; 3) S group (n=30) - SRP and irrigation with Simvastatin. Ten animals from each group were euthanized at 7, 15 and 30 days after treatment. Gingival biopsy specimens were processed to analyze the expression of matrix metalloproteinase 8 (MMP-8). The mandibles were removed and submitted to radiographic and laboratory processing for histometric analysis. The S group showed a significantly lower expression of MMP-8 compared to NT and SRP groups in all experimental periods. In the radiographic and histometric analyses between the groups, S group showed a significantly lower bone loss (BL) compared to NT and SRP groups in all experimental periods. Within the limits of this study, it can be concluded that locally applied statin was effective as an adjuvant treatment for SRP in rats with induced periodontal disease.