Effect of ε-polylysine and ice storage on microbiota composition and quality of Pacific white shrimp (Litopenaeus vannamei) stored at 0 °C
Effect of ε-polylysine and ice storage on microbiota composition and quality of Pacific white shrimp (Litopenaeus vannamei) stored at 0 °C
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DOI：
10.1016/j.fm.2019.04.007
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发表时间：
2019-10-01
影响因子：
5.3
通讯作者：
Luo, Yongkang
中科院分区：
文献类型：
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作者：
Jia, Shiliang;Liu, Yiming;Luo, Yongkang
本研究评估了ε-聚赖氨酸和冰储存对0 ℃下储存的太平洋白色虾的微生物群组成和质量属性的影响。对照组、0.1% ε-聚赖氨酸处理组和冰藏组的货架期分别为5、8和7天。通过Illumina-MiSeq高通量测序靶向16 S rRNA基因探索微生物群组成。在感觉排斥时，假交替单胞菌属，其次是芽孢杆菌属和冷单胞菌属，是第5天腐败对照样品中的优势微生物群。而0.1%的ε-聚赖氨酸则抑制了假交替单胞菌和冷单胞菌的生长。因此，在第8天，在ε-聚赖氨酸处理组中，芽孢杆菌属（Bacilloplama）和弧菌属（Alivibrio）成为优势微生物群。同时，在冰藏样品中，第7天的优势微生物群是Aliivibrio，其次是Moritella和Pseudoalteromonas。此外，由于ε-聚赖氨酸和冰藏对微生物群的调节作用，处理组的化学变化较慢，表现为总挥发性盐基氮、腐胺、尸胺和次黄嘌呤的浓度较低，而肌苷5 '-单磷酸和次黄嘌呤核苷的含量较高。总之，ε-聚赖氨酸和冰储存改变了微生物群组成，并延迟了太平洋白色虾的质量劣化。
This study evaluated the effects of epsilon-Polylysine and ice storage on microbiota composition and quality attributes of Pacific white shrimp stored at 0 degrees C. The sensorial shelf-life of control, 0.1% epsilon-Polylysine treated group, and ice stored group were 5, 8, and 7 days, respectively. Microbiota composition was explored by the Illumina-MiSeq high throughput sequencing targeting of 16S rRNA genes. At the time of sensory rejection, Pseudoalteromonas, followed by Candidatus Bacilloplama and Psychromonas, were the dominant microbiota in spoiled control samples on day 5. However, 0.1% epsilon-Polylysine inhibited the growth of Pseudoalteromonas and Psychromonas. Consequently, Candidatus Bacilloplama followed by Aliivibrio became the dominant microbiota in the epsilon-Polylysine treated group on day 8. Meanwhile, Aliivibrio, followed by Moritella and Pseudoalteromonas were the dominant microbiota in ice stored samples on day 7. Furthermore, due to the modulating effect of epsilon-Polylysine and ice storage on microbiota, chemical changes of the treated groups were slower, which was reflected as lower concentrations of total volatile basic nitrogen, putrescine, cadaverine, and hypoxanthine, and higher contents of inosine 5'-monophosphate and hypoxanthine riboside at the end of storage. In conclusion, epsilon-Polylysine and ice storage altered the microbiota composition and delayed quality deterioration of Pacific white shrimp.