Mediation of the behavioral, endocrine and thermoregulatory actions of ghrelin
Mediation of the behavioral, endocrine and thermoregulatory actions of ghrelin
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DOI：
10.1016/j.yhbeh.2006.03.010
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发表时间：
2006-08-01
影响因子：
3.5
通讯作者：
Telegdy, G.
中科院分区：
文献类型：
--
作者：
Jaszberenyi, M.;Bujdoso, E.;Telegdy, G.
研究了Ghrelin对遥测运动活动的作用，以及该神经肽对自发和探索性运动活动以及相关的内分泌和内稳态参数的影响的传递。侧脑室注射不同剂量的Ghrelin(0.5~5mgGhrelin)可显著增加“开野”装置的正方形穿越活动和站立活动，而遥测记录的自发活动仅在5mgGhrelin剂量时显著增加。Ghrelin还导致皮质酮释放和核心温度显著增加。为了确定这些神经内分泌作用的传递，用不同的拮抗剂，如促肾上腺皮质激素释放激素拮抗剂(α-螺旋CRH9-41)、一氧化氮合酶抑制剂N欧米伽-硝基-L-精氨酸甲酯(L-NAME)、氟哌啶醇、赛庚啶或环氧合酶抑制剂去甲氨基苯那酮(NAP)。开放视野和生物电测结果显示，CRH拮抗剂和氟哌啶醇可使运动反应减弱。在下丘脑-垂体-肾上腺(HPA)激活的情况下，仅赛庚啶预处理被证明有效；氟哌啶醇和L-NAME不改变皮质酮的反应。NAP只有短暂的作用，而赛庚啶对Ghrelin引起的高热反应有更持久的影响；其他拮抗剂被证明无效。目前的数据提示，促肾上腺皮质激素释放和多巴胺能传递可能都参与了Ghrelin引起的行为反应。另一方面，Ghrelin似乎通过5-羟色胺能途径影响HPA的反应，并通过环氧合酶和5-羟色胺能途径影响体温反应。(C)2006 Elsevier Inc.保留所有权利。
The action of ghrelin on telemetrically recorded motor activity and the transmission of the effects of this neuropeptide on spontaneous and exploratory motor activity and some related endocrine and homeostatic parameters were investigated. Different doses (0.5-5 mu g) of ghrelin administered intracerebroventricularly caused significant increases in both square crossing and rearing activity in the "open-field" apparatus, while only the dose of 5 mu g evoked a significant increase in the spontaneous locomotor activity recorded by telemetry. Ghrelin also induced significant increases in corticosterone release and core temperature. To determine the transmission of these neuroendocrine actions, the rats were pretreated with different antagonists, such as a corticotropin-releasing hormone (CRH) antagonist (alpha-helical CRH9-41), the nitric oxide synthase inhibitor N omega-nitro-L-arginine-methyl ester (L-NAME), haloperidol, cyproheptadine or the cyclooxygenase inhibitor noraminophenazone (NAP). The open-field and biotelemetric observations revealed that the motor responses were diminished by pretreatment with the CRH antagonist and haloperidol. In the case of HPA (hypothalamic pituitary adrenal) activation, only cyproheptadine pretreatment proved effective; haloperidol and L-NAME did not modify the corticosterone response. NAP had only a transient, while cyproheptadine elicited a more permanent impact on the hyperthermic response evoked by ghrelin; the other antagonists proved to be ineffective. The present data suggest that both CRH release and dopaminergic transmission may be involved in the ghrelin-evoked behavioral responses. On the other hand, ghrelin appears to have an impact on the HPA response via a serotonergic pathway and on the hyperthermic response via a cyclooxygenase and a serotonergic pathway. (c) 2006 Elsevier Inc. All rights reserved.