"ENDO-BETA-N-ACETYLGLUCOSAMINIDASE-F - ENDOGLYCOSIDASE FROM FLAVOBACTERIUM-MENINGOSEPTICUM THAT CLEAVES BOTH HIGH-MANNOSE AND COMPLEX GLYCOPROTEINS
"ENDO-BETA-N-ACETYLGLUCOSAMINIDASE-F - ENDOGLYCOSIDASE FROM FLAVOBACTERIUM-MENINGOSEPTICUM THAT CLEAVES BOTH HIGH-MANNOSE AND COMPLEX GLYCOPROTEINS
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DOI：
10.1073/pnas.79.15.4540
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
ALEXANDER, S
中科院分区：
文献类型：
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作者：
ELDER, JH;ALEXANDER, S
检测到由脑膜炎败血黄杆菌产生的一种内切糖苷酶活性。这种酶被命名为内切F，它能切割通过天冬酰胺连接到蛋白质主链上的高甘露糖型和复合型聚糖。切割是通过水解与天冬酰胺相邻的N,N'' - 二乙酰壳二糖核心结构的糖苷键进行的，这与内切H和内切D的作用方式类似。在N - 连接糖蛋白中，这种切割位点的可及性存在极大差异。逆转录病毒[劳舍尔鼠白血病病毒]、淋巴细胞性脉络丛脑膜炎病毒、皮钦德病毒以及HLA - A和 - B抗原的糖蛋白在非离子型去污剂存在的情况下很容易被切割。其他的，如卵清蛋白、胎球蛋白、菠萝蛋白酶、卵类黏蛋白、α1 - 酸性糖蛋白、IgG和流感病毒血凝素只有在还原和烷基化之后，或者在1% 2 - 巯基乙醇存在的情况下进行切割时才变得易于切割。内切F应该在将聚糖和蛋白质主链作为独立实体进行研究以及确定聚糖 - 蛋白质界面的性质方面是有用的。
An endoglycosidase activity produced by F. meningosepticum was detected. This enzyme, named endo F, cleaves glycans of both the high-mannose and the complex type linked through asparagine to the protein backbone. Cleavage occurs via hydrolysis of the glycosidic bond of the N,N''-diacetylchitobiose core structure adjacent to asparagine, similar to that due to endo H and endo D. Extreme variability was noted in the availability of this cleavage site among N-linked glycoproteins. Glycoproteins of retrovirus [Rauscher murine leukemia virus], lymphocytic choriomeningitis virus. Pichinde virus, and HLA-A and -B antigens were readily cleaved in the presence of nonionic detergent. Others, such as ovalbumin, fetuin, bromelain, ovomucoid, .alpha.1-acid glycoprotein, IgG, and influenza virus hemagglutinin became susceptible only after reduction and alkylation or when cleavage was performed in the presence of 1% 2-mercaptoethanol. Endo F should prove useful in the study of glycans and protein backbones as discrete entities and for defining the nature of the glycan-protein interface.