Mating-type Distribution and Fertility Status in Magnaporthe grisea Populations from Argentina
Mating-type Distribution and Fertility Status in Magnaporthe grisea Populations from Argentina
复制标题
阿根廷稻瘟病种群的交配型分布和生育力状况
作者：
V. F. Consolo;C. Cordo;Graciela L. Salerno
在阿根廷收集了引起水稻灰斑病的稻瘟病病菌，并对其配种分布和育性进行了分析。在2000年至2003年期间从水稻植株中收集了125株稻瘟病菌分离株。每个分离物通过基于聚合酶链反应的测定来检测交配型。所有阿根廷稻瘟杆菌分离株均属于单一交配型MAT1.1。采用体外对照杂交测定分离株的育性状况，将每个分离株与GUY11和KA9 （MAT1.2标准雌雄同体测试器）配对。由于只有一种可育菌株（KA9）产生周鞘的比例很低（7.2%），因此爆炸病原菌的周鞘产量很少；与其他测试菌株的所有杂交都失败了。未见子囊和子囊孢子。只有一种交配类型的存在和雌性可育分离株的缺失表明，在阿根廷与水稻相关的稻瘟病菌种群中很少或根本没有有性繁殖。
Isolates of Magnaporthe grisea causing gray leaf spot on rice were collected in Argentina and analyzed for mating distribution and fertility. One hundred and twenty-five isolates of M.grisea were collected from rice plants between 2000 and 2003. Each isolate was tested for mating type through a polymerase chain reaction based assay. All M. grisea isolates from Argentina belonged to a single mating type, MAT1.1. The fertility status of isolates was determined using controlled crosses in vitro, pairing each isolate with GUY11 and KA9 (MAT1.2 standard hermaphroditic testers). Production of perithecia was scarce among isolates of the blast pathogen since a low percentage of them (7.2%) developed perithecia with only one of the fertile tester (KA9); all crosses failed with the other tester strain. Asci and ascospores were not observed. The presence of only one mating type and the absence of female fertile isolates indicate that sexual reproduction is rare or absent in M. grisea populations associated with rice in Argentina.